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[1]
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NUCLEOTIDE SEQUENCE [MRNA], BLOCKAGE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY, ENZYME ACTIVITY, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
STRAIN=SAG 1224-5/25;
DOI=10.1074/jbc.M411010200; PubMed=15569691 [NCBI, ExPASy, EBI, Israel, Japan]
Hoffmeister M.,
Piotrowski M.,
Nowitzki U.,
Martin W.;
"Mitochondrial trans-2-enoyl-CoA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis.";
J. Biol. Chem. 280:4329-4338(2005).
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- FUNCTION: Catalyzes reduction of enoyl-CoA to acyl-CoA, an important step of fatty acid biosynthesis which is performed under anaerobiosis. Under anaeobiosis, mitochondria perform a malonyl-CoA independent synthesis of fatty acids leading to accumulation of wax esters, which serve as the sink for electrons stemming from glycolytic ATP synthesis and pyruvate oxidation. Can use both NADH and NADPH as electron donor, with 2-3-fold higher specific activities for NADH relative to NADPH.
- CATALYTIC ACTIVITY: Acyl-CoA + NAD+ = trans-didehydroacyl-CoA + NADH.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=68 µM for NADH; | | KM=119 µM for NADPH; | | KM=91 µM for trans-2-hexenoyl-CoA; | |
- SUBUNIT: Monomer.
- SUBCELLULAR LOCATION: Mitochondrion.
- DEVELOPMENTAL STAGE: Expressed under aerobic and anaerobic conditions.
- PTM: The N-terminus is blocked.
- SIMILARITY: Belongs to the TER reductase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 539 AA [This is the length of the unprocessed precursor] |
Molecular weight: 57303 Da [This is the MW of the unprocessed precursor] |
CRC64: A52C244AC61E2CDF [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSCPASPSAA VVSAGALCLC VATVLLATGS NPTALSTAST RSPTSLVRGV DRGLMRPTTA
70 80 90 100 110 120
AALTTMREVP QMAEGFSGEA TSAWAAAGPQ WAAPLVAAAS SALALWWWAA RRSVRRPLAA
130 140 150 160 170 180
LAELPTAVTH LAPPMAMFTT TAKVIQPKIR GFICTTTHPI GCEKRVQEEI AYARAHPPTS
190 200 210 220 230 240
PGPKRVLVIG CSTGYGLSTR ITAAFGYQAA TLGVFLAGPP TKGRPAAAGW YNTVAFEKAA
250 260 270 280 290 300
LEAGLYARSL NGDAFDSTTK ARTVEAIKRD LGTVDLVVYS IAAPKRTDPA TGVLHKACLK
310 320 330 340 350 360
PIGATYTNRT VNTDKAEVTD VSIEPASPEE IADTVKVMGG EDWELWIQAL SEAGVLAEGA
370 380 390 400 410 420
KTVAYSYIGP EMTWPVYWSG TIGEAKKDVE KAAKRITQQY GCPAYPVVAK ALVTQASSAI
430 440 450 460 470 480
PVVPLYICLL YRVMKEKGTH EGCIEQMVRL LTTKLYPENG APIVDEAGRV RVDDWEMAED
490 500 510 520 530
VQQAVKDLWS QVSTANLKDI SDFAGYQTEF LRLFGFGIDG VDYDQPVDVE ADLPSAAQQ
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Q5EU90 in FASTA format |
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