ID   CRD1_HORVU              Reviewed;         417 AA.
AC   Q5EFU4;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   04-NOV-2008, entry version 22.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic;
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
DE   AltName: Full=Protein Xantha-l;
DE   Flags: Precursor;
GN   Name=CRD1;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Pooideae; Triticeae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY, PATHWAY, FUNCTION,
RP   SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-154 AND SER-180.
RX   PubMed=15824317; DOI=10.1073/pnas.0501784102;
RA   Rzeznicka K., Walker C.J., Westergren T., Kannangara C.G.,
RA   von Wettstein D., Merchant S., Gough S.P., Hansson M.;
RT   "Xantha-l encodes a membrane subunit of the aerobic Mg-protoporphyrin
RT   IX monomethyl ester cyclase involved in chlorophyll biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:5886-5891(2005).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in
CC       chlorophyll biosynthesis. Mediates the cyclase reaction, which
CC       results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin
CC       IX 13-monomethyl ester (MgPMME).
CC   -!- CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl
CC       ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX
CC       13-monomethyl ester + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-hydroxy-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-
CC       protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-oxo-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide +
CC       NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane; Peripheral
CC       membrane protein (Potential).
CC   -!- SIMILARITY: Belongs to the acsF family.
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DR   EMBL; AY887063; AAW80518.1; -; Genomic_DNA.
DR   Gramene; Q5EFU4; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl este...; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   1: Evidence at protein level;
KW   Chlorophyll biosynthesis; Chloroplast; Iron; Membrane; Metal-binding;
KW   NADP; Oxidoreductase; Photosynthesis; Plastid; Transit peptide.
FT   TRANSIT       1     45       Chloroplast (Potential).
FT   CHAIN        46    417       Magnesium-protoporphyrin IX monomethyl
FT                                ester [oxidative] cyclase, chloroplastic.
FT                                /FTId=PRO_0000000603.
FT   MUTAGEN     154    154       G->E: In xantha-l(81); induces
FT                                accumulation of MgPMME.
FT   MUTAGEN     180    180       S->F: In xantha-l(35); induces
FT                                accumulation of MgPMME.
SQ   SEQUENCE   417 AA;  48161 MW;  83933398C325E77E CRC64;
     MASAMELSLL NPAMHHYGIA AKTASHLPVV PARRASSGAV RFRVRAAAAA PPAPAAKPGS
     PKKRGKTEVN ESLLTPRFYT TDFDEMEQLF NAEINKQLNQ DEFDALLQEF KTDYNQTHFI
     RNPEFKEAAD KMQGPLRQIF VEFLERSCTA EFSGFLLYKE LGRRLKKTNP VVAEIFSLMS
     RDEARHAGFL NKGLSDFNLA LDLGFLTKAR KYTFFKPKFI FYATYLSEKI GYWRYITIFR
     HLKANPEYQV YPIFKYFENW CQDENRHGDF FSALLKAQPQ FLNDWKAKLW SRFFCLSVYI
     TMYLNDCQRS AFYEGIGLNT KEFDMHVIYE TNRTTARIFP AVPDVENPEF KRKLDRMVDI
     NLKIISIGES NDLPLVKNLK RVPLIAQLVS EIIAAYLMPP IESGSVDFAE FEPKLVY
//