ID   CYSI_VIBF1              Reviewed;         576 AA.
AC   Q5E840;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-NOV-2008, entry version 24.
DE   RecName: Full=Sulfite reductase [NADPH] hemoprotein beta-component;
DE            Short=SIR-HP;
DE            Short=SIRHP;
DE            EC=1.8.1.2;
GN   Name=cysI; OrderedLocusNames=VF_0311;
OS   Vibrio fischeri (strain ATCC 700601 / ES114).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium
RT   with pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: This enzyme catalyzes the 6-electron reduction of
CC       sulfite to sulfide. This is one of several activities required for
CC       the biosynthesis of L-cysteine from sulfate (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3
CC       NADPH.
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein,
CC       the beta component is a hemoprotein (By similarity).
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000020; AAW84806.1; -; Genomic_DNA.
DR   RefSeq; YP_203694.1; -.
DR   GeneID; 3277269; -.
DR   GenomeReviews; CP000020_GR; VF_0311.
DR   KEGG; vfi:VF0311; -.
DR   NMPDR; fig|312309.3.peg.311; -.
DR   HOGENOM; Q5E840; -.
DR   BioCyc; VFIS312309:VF0311-MON; -.
DR   GO; GO:0009337; C:sulfite reductase complex (NADPH); IEA:InterPro.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0004783; F:sulfite reductase (NADPH) activity; IEA:HAMAP.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:HAMAP.
DR   HAMAP; MF_01540; -; 1.
DR   InterPro; IPR011786; CysI.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR006067; Nir_Sir_4Fe4S.
DR   InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR   Pfam; PF01077; NIR_SIR; 1.
DR   Pfam; PF03460; NIR_SIR_ferr; 2.
DR   PRINTS; PR00397; SIROHAEM.
DR   TIGRFAMs; TIGR02041; CysI; 1.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Amino-acid biosynthesis; Complete proteome;
KW   Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    576       Sulfite reductase [NADPH] hemoprotein
FT                                beta-component.
FT                                /FTId=PRO_0000199913.
FT   METAL       439    439       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       445    445       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       485    485       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       489    489       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL       489    489       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   576 AA;  64631 MW;  DEC6E859BAF9FD3C CRC64;
     MSELIDVKTI LSGTELGPLA DNERLKRESK YLRGTIVEDL QDRITGGFTK DNFQLIRFHG
     MYQQDDRDIR AERAKQKLEP LHNVMLRARM PGGIITPKQW LAIDKFAEEH TSYGSLRLTT
     RQTFQFHGVL KPNIKLMHQT LNSIGIDSIA TAGDVNRNVL CTTNPVESEL HQEAYEWAKK
     ISEHLLPKTR AYAEIWLDGE KLETTDEEPI LGSNYLPRKF KTTVVIPPQN DVDVHANDLN
     FIAIADNGKL VGFNVLVGGG LAMTHGDTAT YPRKADDFGF VPLDKTLDVA AAVVTTQRDW
     GNRSNRKNAK TKYTLDRVGS DVFKGEVEKR AGVQFEKSRP YELTERGDRI GWVEGIDGKH
     HLALFIENGR LLDYPGKPLK TGVAEIAKVH QGDFRMTANQ NLIVAGVPSE QKDHIEQIAR
     AHGLIDDTHS EQRKNSMACV AFPTCPLAMA EAERFLPEFV TEIEGVLKKH NLPEEDNIIF
     RVTGCPNGCG RAMLAEIGLV GKAPGRYNFH LGGNRSGTRV PKMYKENITD RQILTEIDQL
     VGRWATERNE NEGFGDFTIR AGIVDEVKVS KRDFHA
//