NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
DOI=10.1016/0167-4781(95)00018-C; PubMed=7711073 [NCBI, ExPASy, EBI, Israel, Japan]
Arai H., Igarashi Y., Kodama T.;
"The structural genes for nitric oxide reductase from Pseudomonas aeruginosa.";
Biochim. Biophys. Acta 1261:279-284(1995).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
DOI=10.1038/35023079; PubMed=10984043 [NCBI, ExPASy, EBI, Israel, Japan]
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.";
Nature 406:959-964(2000).

Comments

FUNCTION: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen reduction is probably coupled to electron transport phosphorylation (By similarity).
CATALYTIC ACTIVITY: Nitrous oxide + acceptor + H₂O = 2 nitric oxide + reduced acceptor.
PATHWAY: Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 3/4 .
SUBUNIT: Heterodimer of cytochromes b (large subunit) and c (small subunit).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the heme-copper respiratory oxidase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D38133; BAA07330.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE004091; AAG03913.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S53713; S53713.

RefSeq

NP_249215.1; -.

3D structure databases

ModBase

Q59647.

Enzyme and pathway databases

BioCyc

PAER208964:PA0524-MON; -.

Organism-specific databases

PseudoCAP

PA0524; -.

Family and domain databases

InterPro

IPR000883; COX1.
Graphical view of domain structure.

Gene3D

G3DSA:1.20.210.10; COX1; 1.

Pfam

PF00115; COX1; 1.
Pfam graphical view of domain structure.

PROSITE

PS50855; COX1; 1.
PS00077; COX1_CUB; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q59647.

Genome annotation databases

882193; -.

AE004091_GR; PA0524.

pae:PA0524; -.

fig|208964.1.peg.525; -.

Phylogenomic databases

HOGENOM

Q59647; -.

Genome annotation databases

CMR

Q59647; PA0524.

Other

ProtoNet

Q59647.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Electron transport; Heme; Iron; Membrane; Metal-binding; Oxidoreductase; Respiratory chain; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 466`	`466`	`Nitric oxide reductase subunit B.`	`PRO_0000183472`
`TRANSMEM`	`19 39`	`21`	`Potential.`
`TRANSMEM`	`61 81`	`21`	`Potential.`
`TRANSMEM`	`95 115`	`21`	`Potential.`
`TRANSMEM`	`142 162`	`21`	`Potential.`
`TRANSMEM`	`169 189`	`21`	`Potential.`
`TRANSMEM`	`205 225`	`21`	`Potential.`
`TRANSMEM`	`243 263`	`21`	`Potential.`
`TRANSMEM`	`270 290`	`21`	`Potential.`
`TRANSMEM`	`308 328`	`21`	`Potential.`
`TRANSMEM`	`349 369`	`21`	`Potential.`
`TRANSMEM`	`391 411`	`21`	`Potential.`
`TRANSMEM`	`434 454`	`21`	`Potential.`
`METAL`	`60 60`		`Iron (low-spin heme axial ligand) (Probable).`
`METAL`	`207 207`		`Iron B (Probable).`
`METAL`	`258 258`		`Iron B (Probable).`
`METAL`	`259 259`		`Iron B (Probable).`
`METAL`	`348 348`		`Iron (high-spin heme axial ligand) (Probable).`
`METAL`	`350 350`		`Iron (low-spin heme axial ligand) (Probable).`

Sequence information

Length: 466 AA [This is the length of the unprocessed precursor]

Molecular weight: 52336 Da [This is the MW of the unprocessed precursor]

CRC64: DFB379172C86D5B7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMSPNGSLKF ASQAVAKPYF VFALILFVGQ ILFGLIMGLQ YVVGDFLFPA IPFNVARMVH 

        70         80         90        100        110        120 
TNLLIVWLLF GFMGAAYYLV PEESDCELYS PKLAWILFWV FAAAGVLTIL GYLLVPYAGL 

       130        140        150        160        170        180 
ARLTGNELWP TMGREFLEQP TISKAGIVIV ALGFLFNVGM TVLRGRKTAI SMVLMTGLIG 

       190        200        210        220        230        240 
LALLFLFSFY NPENLTRDKF YWWWVVHLWV EGVWELIMGA ILAFVLVKIT GVDREVIEKW 

       250        260        270        280        290        300 
LYVIIAMALI SGIIGTGHHY FWIGVPGYWL WLGSVFSALE PLPFFAMVLF AFNTINRRRR 

       310        320        330        340        350        360 
RDYPNRAVAL WAMGTTVMAF LGAGVWGFMH TLAPVNYYTH GTQLTAAHGH MAFYGAYAMI 

       370        380        390        400        410        420 
VMTIISYAMP RLRGIGEAMD NRSQVLEMWG FWLMTVAMVF ITLFLSAAGV LQVWLQRMPA 

       430        440        450        460 
DGAAMTFMAT QDQLAIFYWL REGAGVVFLI GLVAYLLSFR RGKAAA

Q59647 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools