NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8575452 [NCBI, ExPASy, EBI, Israel, Japan]
Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R.;
"The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide.";
Eur. J. Biochem. 234:910-920(1995).

Comments

FUNCTION: Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). Can only oxidize formylmethanofuran. This enzyme is oxygen-labile.
CATALYTIC ACTIVITY: Formylmethanofuran + H₂O + acceptor = CO₂ + methanofuran + reduced acceptor.
COFACTOR: Tungsten.
PATHWAY: One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3 .
SUBUNIT: This enzyme is composed of seven subunits fwdA (65 kDa), fwdB (53 kDa), fwdC (31 kDa), fwdD (15 kDa), fwdE, fwdF and fwdG (By similarity).
INDUCTION: By growth on tungsten or molybdenum under anaerobic conditions.
SIMILARITY: Belongs to the fwdC/fmdC family.

Copyright

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Cross-references

Sequence databases

EMBL

X87970; CAA61214.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q59579.

Family and domain databases

InterPro

IPR017550; Formylmethanofuran_DH_C.
IPR002489; Glu_synthase_C.
Graphical view of domain structure.

Gene3D

G3DSA:2.160.20.60; Glu_synthase_C; 1.

Pfam

PF01493; GXGXG; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methanogenesis; Oxidoreductase; Repeat; Tungsten.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 270`	`270`	`Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C.`	`PRO_0000144197`
`REPEAT`	`80 92`	`13`	`1.`
`REPEAT`	`99 111`	`13`	`2.`
`REPEAT`	`118 130`	`13`	`3.`
`REPEAT`	`144 156`	`13`	`4.`
`REPEAT`	`163 175`	`13`	`5.`
`REPEAT`	`182 194`	`13`	`6.`
`REPEAT`	`201 213`	`13`	`7.`
`REGION`	`80 213`	`134`	`7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-[IL]-X-[IV]-X-G.`

Sequence information

Length: 270 AA [This is the length of the unprocessed precursor]

Molecular weight: 28555 Da [This is the MW of the unprocessed precursor]

CRC64: E0A369D2ACFEC46F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSEIILTPKE QPEVPLEAPN IKPDVFAGKS IDEIKNIQIM YGNEVVKLGD FFEVSGEPAD 

        70         80         90        100        110        120 
AASDIKIIID GDVYNTKRIG QEMTAGEILV KGNVNMYVGA GMKGGRITVE GNAASWAGQD 

       130        140        150        160        170        180 
MRGGELEILG NAADYVGSSY RGDWRGMSGG VITVHGNAGN EIGEYMNGGK IIIKGDVNIM 

       190        200        210        220        230        240 
PGIHMNNGLI IIEGNAVARV GGEMAGGTII VKGMIQEFLP GFTYLGVEKD IEVNGETFPG 

       250        260        270 
AFYKFEGDHA IKGAKGIVYA AVGCNGHIEP

Q59579 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools