NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DSM 5473;
PubMed=7642512 [NCBI, ExPASy, EBI, Israel, Japan]
Kletzin A., Mukund S., Kelley-Crouse T.L., Chan M.K.S., Rees D.C., Adams M.W.W.;
"Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis.";
J. Bacteriol. 177:4817-4819(1995).

[2]

PROTEIN SEQUENCE OF 1-26, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND CHARACTERIZATION.
STRAIN=DSM 5473;
PubMed=8390467 [NCBI, ExPASy, EBI, Israel, Japan]
Mukund S., Adams M.W.W.;
"Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism.";
J. Biol. Chem. 268:13592-13600(1993).

Comments

FUNCTION: Catalyzes the oxidation of C1-C3 aldehydes. Involved in peptides fermentation.
CATALYTIC ACTIVITY: An aldehyde + H₂O + 2 oxidized ferredoxin = an acid + 2 H⁺ + 2 reduced ferredoxin.
COFACTOR: Binds 1 4Fe-4S cluster per subunit.
COFACTOR: Binds 1 tungstopterin cofactor per subunit.
ENZYME REGULATION: Inhibited by arsenite, iodoacetate and cyanide.

BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters:	K_M=62 mM for formaldehyde;
	V_max=17.5 µmol/min/mg enzyme with formaldehyde as substrate;
	Note=Significant activity only with aliphatic aldehydes up to C3 and only at high concentration (15 mM);
pH dependence:	Optimum pH is above 10 (at 80 degrees Celsius);
Temperature dependence:	Optimum temperature is above 90 degrees Celsius (at pH 8.4);

SUBUNIT: Homotetramer.
MISCELLANEOUS: A low amount of aldehyde ferredoxin oxidoreductase activity has been observed.
SIMILARITY: Belongs to the AOR/FOR family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X83963; CAA58795.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

O93738; 1B25. [HSSP ENTRY / PDB]

SMR

Q56303; 1-621.

ModBase

Q56303.

Family and domain databases

InterPro

IPR013985; Ald_Fedxn_OxRdtase_3.
IPR013983; Ald_Fedxn_OxRdtase_N.
IPR001203; OxRdtase_Ald_Fedxn_C.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.599.10; Oxred_Ald_Fedxn_3; 1.
G3DSA:3.60.9.10; Oxred_Ald_Fedxn_N; 1.

Pfam

PF01314; AFOR_C; 1.
PF02730; AFOR_N; 1.
Pfam graphical view of domain structure.

SMART

SM00790; AFOR_N; 1.
SMART graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Tungsten.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 621`	`621`	`Tungsten-containing formaldehyde ferredoxin oxidoreductase.`	`PRO_0000087325`
`METAL`	`284 284`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`287 287`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`291 291`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`493 493`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 621 AA [This is the length of the unprocessed precursor]

Molecular weight: 68941 Da [This is the MW of the unprocessed precursor]

CRC64: 95589C2DC4F18E13 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKGWWGKILR VDLTNNKVWV QEYSPEVAKN FIGGRGLAAW ILWNEAKNVD PLGPKNKLVF 

        70         80         90        100        110        120 
ATGPFNGLPT PSGGKMVIAA KSPLTGGYGD GNLGTMATVH LRKAGYDALV VEGKAKKPVY 

       130        140        150        160        170        180 
IYIEDDNVSI LSAEGLWGKT TFETERELKE IHGKNVGILS IGPGGENLVK YAVVISQEGR 

       190        200        210        220        230        240 
AAGRPGMGAV MGSKKLKAVV IKGTKEIPVA DKEKLKELSQ EAYDAILNSP GYPFWHRQGT 

       250        260        270        280        290        300 
MAAVEWTNEN SALPTRNFSD GSFEFARSID GYTMEGMKVK QRGCPYCNMP CGNVVLDAEG 

       310        320        330        340        350        360 
QESELDYENV ALLGSNLGIG KLNEVSVLNR IADEMGLDTI SLGVAISYVM EAKEKGIIKD 

       370        380        390        400        410        420 
DDAPEFGDFK KAKQLALDIA YRRGELGNLA AEGVKVMSEK LGAKDFAMHV KGLEVSGYNC 

       430        440        450        460        470        480 
YIYPAMALAY GTSSIGAHHK EAWVIAWEIG TAPIEGEKAQ KVEYKITYDP EKAAKVIELQ 

       490        500        510        520        530        540 
RLRGGLFEML TACRLPWVEV GLSLDYYPKL LEAITGVKYT WDDLYKAADR VYALMRAYWV 

       550        560        570        580        590        600 
REFNGNWSRE MDYPPERWFK EGLKSGPYKG QHLEKDKYDA LLSEYYKLRG WDERGIPKKE 

       610        620 
TLKELNLEFV IPELEKVTKL E

Q56303 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools