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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1074/jbc.272.7.4201; PubMed=9020134 [NCBI, ExPASy, EBI, Israel, Japan]
Yano T.,
Chu S.S.,
Sled' V.D.,
Ohnishi T.,
Yagi T.;
"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit.";
J. Biol. Chem. 272:4201-4211(1997).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Masui R.,
Kurokawa K.,
Nakagawa N.,
Tokunaga F.,
Koyama Y.,
Shibata T.,
Oshima T.,
Yokoyama S.,
Yasunaga T.,
Kuramitsu S.;
"Complete genome sequence of Thermus thermophilus HB8.";
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
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- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
- CATALYTIC ACTIVITY: NADH + quinone = NAD+ + quinol.
- SUBUNIT: NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
- SIMILARITY: Belongs to the complex I subunit 2 family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 427 AA [This is the length of the unprocessed precursor] |
Molecular weight: 44436 Da [This is the MW of the unprocessed precursor] |
CRC64: 9AD943608E5C2759 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MTLAILAVFS VALTLLGFVL PPQGVKRATL LGLALALASL LLTWGKPFAF GPYAVDGVSQ
70 80 90 100 110 120
VFTLLALLGA LWTVGLVRSG RFEFYLLVLY AALGMHLLAS TRHLLLMLVA LEALSLPLYA
130 140 150 160 170 180
LATWRRGQGL EAALKYFLLG ALAAAFFLYG AALFYGATGS LVLGAPGEGP LYALALGLLL
190 200 210 220 230 240
VGLGFKAALA PFHFWTPDVY QGSPTPVVLF MATSVKAAAF AALLRVAAPP EALALLVALS
250 260 270 280 290 300
VVVGNLAALA QKEAKRLLAY SSIAHAGYMA LALYTGNAQA LGFYLLTYVL ATGLAFAVLS
310 320 330 340 350 360
QISPDRVPLE ALRGLYRKDP LLGLAFLVAM LSLLGLPPLA GFWGKYLAFA EAARAGAWGV
370 380 390 400 410 420
LVLALVTSAV SAYYYLGLGL AVFARPEETP FRPGPPWARA AVVAAGVLLL ALGLLPGLVL
PALAAGG
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Q56229 in FASTA format |
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