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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1074/jbc.272.7.4201; PubMed=9020134 [NCBI, ExPASy, EBI, Israel, Japan]
Yano T.,
Chu S.S.,
Sled' V.D.,
Ohnishi T.,
Yagi T.;
"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit.";
J. Biol. Chem. 272:4201-4211(1997).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Masui R.,
Kurokawa K.,
Nakagawa N.,
Tokunaga F.,
Koyama Y.,
Shibata T.,
Oshima T.,
Yokoyama S.,
Yasunaga T.,
Kuramitsu S.;
"Complete genome sequence of Thermus thermophilus HB8.";
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
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[3]
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PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
DOI=10.1021/bi0600998; PubMed=16584177 [NCBI, ExPASy, EBI, Israel, Japan]
Hinchliffe P.,
Carroll J.,
Sazanov L.A.;
"Identification of a novel subunit of respiratory complex I from Thermus thermophilus.";
Biochemistry 45:4413-4420(2006).
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[4]
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X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, AND ELECTRON TRANSFER MECHANISM.
DOI=10.1126/science.1123809; PubMed=16469879 [NCBI, ExPASy, EBI, Israel, Japan]
Sazanov L.A.,
Hinchliffe P.;
"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus.";
Science 311:1430-1436(2006).
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- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo4 subunit may contain the quinone-binding site.
- CATALYTIC ACTIVITY: NADH + quinone = NAD+ + quinol.
- SUBUNIT: NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo4 interacts extensively with nqo6.
- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side.
- SIMILARITY: Belongs to the complex I 49 kDa subunit family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 409 AA [This is the length of the unprocessed precursor] |
Molecular weight: 46371 Da [This is the MW of the unprocessed precursor] |
CRC64: 161AA0C796D62ED3 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MREEFLEEIP LDAPPEEAKE LRTEVMTLNV GPQHPSTHGV LRLMVTLSGE EVLEVVPHIG
70 80 90 100 110 120
YLHTGFEKTM EHRTYLQNIT YTPRMDYLHS FAHDLAYALA VEKLLGAVVP PRAETIRVIL
130 140 150 160 170 180
NELSRLASHL VFLGTGLLDL GALTPFFYAF RERETILDLF EWVTGQRFHH NYIRIGGVKE
190 200 210 220 230 240
DLPEEFVPEL KKLLEVLPHR IDEYEALFAE SPIFYERARG VGVIPPEVAI DLGLTGGSLR
250 260 270 280 290 300
ASGVNYDVRK AYPYSGYETY TFDVPLGERG DVFDRMLVRI REMRESVKII KQALERLEPG
310 320 330 340 350 360
PVRDPNPQIT PPPRHLLETS MEAVIYHFKH YTEGFHPPKG EVYVPTESAR GELGYYIVSD
370 380 390 400
GGSMPYRVKV RAPSFVNLQS LPYACKGEQV PDMVAIIASL DPVMGDVDR
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Q56220 in FASTA format |
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