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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1074/jbc.272.7.4201; PubMed=9020134 [NCBI, ExPASy, EBI, Israel, Japan]
Yano T.,
Chu S.S.,
Sled' V.D.,
Ohnishi T.,
Yagi T.;
"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit.";
J. Biol. Chem. 272:4201-4211(1997).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Masui R.,
Kurokawa K.,
Nakagawa N.,
Tokunaga F.,
Koyama Y.,
Shibata T.,
Oshima T.,
Yokoyama S.,
Yasunaga T.,
Kuramitsu S.;
"Complete genome sequence of Thermus thermophilus HB8.";
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
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[3]
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PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY, EPR SPECTROSCOPY, AND SUBUNIT.
DOI=10.1021/bi0600998; PubMed=16584177 [NCBI, ExPASy, EBI, Israel, Japan]
Hinchliffe P.,
Carroll J.,
Sazanov L.A.;
"Identification of a novel subunit of respiratory complex I from Thermus thermophilus.";
Biochemistry 45:4413-4420(2006).
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[4]
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X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, AND ELECTRON TRANSFER MECHANISM.
DOI=10.1126/science.1123809; PubMed=16469879 [NCBI, ExPASy, EBI, Israel, Japan]
Sazanov L.A.,
Hinchliffe P.;
"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus.";
Science 311:1430-1436(2006).
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- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
- CATALYTIC ACTIVITY: NADH + quinone = NAD+ + quinol.
- COFACTOR: Binds 1 4Fe-4S cluster per subunit. This 4Fe-4S cluster is referred to as N2.
- SUBUNIT: NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo6 interacts extensively with nqo4.
- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side.
- SIMILARITY: Belongs to the complex I 20 kDa subunit family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 181 AA [This is the length of the unprocessed precursor] |
Molecular weight: 20239 Da [This is the MW of the unprocessed precursor] |
CRC64: A1E030366E6692F1 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MALKDLFERD VQELEREGIL FTTLEKLVAW GRSNSLWPAT FGLACCAIEM MASTDARNDL
70 80 90 100 110 120
ARFGSEVFRA SPRQADVMIV AGRLSKKMAP VMRRVWEQMP DPKWVISMGA CASSGGMFNN
130 140 150 160 170 180
YAIVQNVDSV VPVDVYVPGC PPRPEALIYA VMQLQKKVRG QAYNERGERL PPVAAWKRTR
G
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Q56218 in FASTA format |
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