ID   IDHA_DICDI              Reviewed;         354 AA.
AC   Q55BI2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-NOV-2008, entry version 23.
DE   RecName: Full=Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial;
DE            EC=1.1.1.41;
DE   Flags: Precursor;
GN   Name=idhA; ORFNames=DDB_G0271344;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   MEDLINE=22092622; PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A.,
RA   Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G.,
RA   Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A.,
RA   Platzer M., Rosenthal A., Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Performs an essential role in the oxidative function of
CC       the citric acid cycle (By similarity).
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) +
CC       NADH.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- SUBUNIT: Heteroligomer of catalytic and regulatory subunits (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family.
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DR   EMBL; AAFI02000006; EAL71802.1; -; Genomic_DNA.
DR   RefSeq; XP_645630.1; -.
DR   GeneID; 3396844; -.
DR   KEGG; ddi:DDB_0231288; -.
DR   dictyBase; DDB_G0271344; idhA.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0004449; F:isocitrate dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006102; P:isocitrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   InterPro; IPR004434; IsoCit_DHase_NAD_mit.
DR   InterPro; IPR001804; IsoCit_IM_DHase.
DR   InterPro; IPR010916; TonB_box_CS.
DR   Gene3D; G3DSA:3.40.718.10; IDH_IMDH; 1.
DR   PANTHER; PTHR11835; IDH_IMDH_dimeric; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   TIGRFAMs; TIGR00175; mito_nad_idh; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Magnesium; Manganese; Metal-binding; Mitochondrion;
KW   NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    354       Isocitrate dehydrogenase [NAD] regulatory
FT                                subunit A, mitochondrial.
FT                                /FTId=PRO_0000328018.
FT   NP_BIND     276    282       NADP (By similarity).
FT   METAL       243    243       Magnesium or manganese (By similarity).
FT   BINDING      95     95       Substrate (By similarity).
FT   BINDING      97     97       Substrate (By similarity).
FT   BINDING     101    101       Substrate (By similarity).
FT   BINDING     111    111       Substrate (By similarity).
FT   BINDING     132    132       Substrate (By similarity).
FT   BINDING     289    289       NADP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   SITE        139    139       Critical for catalysis (By similarity).
FT   SITE        186    186       Critical for catalysis (By similarity).
SQ   SEQUENCE   354 AA;  38341 MW;  07BC0DC939425EBE CRC64;
     MFSRKSLSIF STLRNYSSST SKIQKVTLIP GDGIGPEISE SVKRVFSAVK APIEWETVVV
     DANTGISKEV IESISKNKIG LKGPISTPIG TGHQSLNLGL RKTFNLYANI RPCLSIPGHK
     TRYNNVNTVV VRENTEGEYS GIENQPVKGV AQSIKIITKE ASTRIAHYAF QYALANGRKK
     VTCIHKANIM KQSDGLFVKS CREVSTRYPS IKYEELTIDN NCMQLVLDPN QMDVMVLPNL
     YGDIVSDLCA GLIGGLGLTP SGNIGENGSA IFEAVHGTAP DIAGKNKANP TALILSSIMM
     LRHLGHFHEA SIIENAVLNT LTEGKVKTGD LGGNSSCSEY TDELVKKITE SLNK
//