ID   HPPD_STRAW              Reviewed;         381 AA.
AC   Q53586; Q93HN6;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   09-MAY-2003, sequence version 2.
DT   04-NOV-2008, entry version 66.
DE   RecName: Full=4-hydroxyphenylpyruvate dioxygenase;
DE            Short=HPPDase;
DE            Short=4HPPD;
DE            Short=HPD;
DE            EC=1.13.11.27;
GN   Name=hpd; OrderedLocusNames=SAV_5149;
OS   Streptomyces avermitilis.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=33903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31272 / MA-4848;
RX   MEDLINE=94350811; PubMed=8071207;
RA   Denoya C.D., Skinner D.D., Morgenstern M.R.;
RT   "A Streptomyces avermitilis gene encoding a 4-hydroxyphenylpyruvic
RT   acid dioxygenase-like protein that directs the production of
RT   homogentisic acid and an ochronotic pigment in Escherichia coli.";
RL   J. Bacteriol. 176:5312-5319(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165;
RX   MEDLINE=21477403; PubMed=11572948; DOI=10.1073/pnas.211433198;
RA   Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C.,
RA   Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T.,
RA   Kikuchi H., Shiba T., Sakaki Y., Hattori M.;
RT   "Genome sequence of an industrial microorganism Streptomyces
RT   avermitilis: deducing the ability of producing secondary
RT   metabolites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165;
RX   MEDLINE=22608306; PubMed=12692562; DOI=10.1038/nbt820;
RA   Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M., Omura S.;
RT   "Complete genome sequence and comparative analysis of the industrial
RT   microorganism Streptomyces avermitilis.";
RL   Nat. Biotechnol. 21:526-531(2003).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), METAL-BINDING SITES, AND
RP   SUBUNIT.
RX   PubMed=15157070; DOI=10.1021/bi049317s;
RA   Brownlee J.M., Johnson-Winters K., Harrison D.H.T., Moran G.R.;
RT   "Structure of the ferrous form of (4-hydroxyphenyl)pyruvate
RT   dioxygenase from Streptomyces avermitilis in complex with the
RT   therapeutic herbicide, NTBC.";
RL   Biochemistry 43:6370-6377(2004).
CC   -!- CATALYTIC ACTIVITY: 4-hydroxyphenylpyruvate + O(2) = homogentisate
CC       + CO(2).
CC   -!- COFACTOR: Binds 1 iron ion per subunit.
CC   -!- PATHWAY: Amino-acid degradation; L-phenylalanine degradation;
CC       acetoacetic acid and fumarate from L-phenylalanine: step 3/6.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the 4HPPD family.
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DR   EMBL; U11864; AAA50231.1; -; Genomic_DNA.
DR   EMBL; AB070935; BAB69150.1; -; Genomic_DNA.
DR   EMBL; BA000030; BAC72861.1; -; Genomic_DNA.
DR   RefSeq; NP_826326.1; -.
DR   PDB; 1T47; X-ray; 2.50 A; A/B=1-381.
DR   PDBsum; 1T47; -.
DR   GeneID; 1211215; -.
DR   GenomeReviews; BA000030_GR; SAV_5149.
DR   KEGG; sma:SAV5149; -.
DR   NMPDR; fig|227882.1.peg.5152; -.
DR   HOGENOM; Q53586; -.
DR   BioCyc; SAVE227882:SAV5149-MON; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006572; P:tyrosine catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR005956; 4OHPhenylPyrv_dOase.
DR   InterPro; IPR004360; Glyas_bleo-R_dOase.
DR   PANTHER; PTHR11959; HPP_dOase; 1.
DR   Pfam; PF00903; Glyoxalase; 2.
DR   PIRSF; PIRSF009283; HPP_dOase; 1.
DR   TIGRFAMs; TIGR01263; 4HPPD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Dioxygenase; Iron; Metal-binding;
KW   Oxidoreductase; Phenylalanine catabolism; Tyrosine catabolism.
FT   CHAIN         1    381       4-hydroxyphenylpyruvate dioxygenase.
FT                                /FTId=PRO_0000088409.
FT   METAL       187    187       Iron.
FT   METAL       270    270       Iron.
FT   METAL       349    349       Iron.
FT   CONFLICT     40     40       Missing (in Ref. 1; AAA50231).
FT   STRAND       22     29
FT   HELIX        33     42
FT   STRAND       47     53
FT   HELIX        54     56
FT   STRAND       60     68
FT   STRAND       71     80
FT   HELIX        84     96
FT   STRAND       98    106
FT   HELIX       110    119
FT   STRAND      124    133
FT   STRAND      136    144
FT   STRAND      150    159
FT   STRAND      161    164
FT   STRAND      184    191
FT   HELIX       197    208
FT   HELIX       219    222
FT   TURN        223    226
FT   STRAND      227    234
FT   STRAND      240    247
FT   STRAND      250    252
FT   HELIX       255    263
FT   STRAND      267    274
FT   HELIX       278    287
FT   HELIX       297    299
FT   HELIX       303    307
FT   HELIX       314    320
FT   STRAND      323    326
FT   STRAND      331    337
FT   STRAND      340    344
FT   STRAND      347    355
FT   HELIX       363    376
SQ   SEQUENCE   381 AA;  41863 MW;  4A3B3E7B646165D7 CRC64;
     MTQTTHHTPD TARQADPFPV KGMDAVVFAV GNAKQAAHYY STAFGMQLVA YSGPENGSRE
     TASYVLTNGS ARFVLTSVIK PATPWGHFLA DHVAEHGDGV VDLAIEVPDA RAAHAYAIEH
     GARSVAEPYE LKDEHGTVVL AAIATYGKTR HTLVDRTGYD GPYLPGYVAA APIVEPPAHR
     TFQAIDHCVG NVELGRMNEW VGFYNKVMGF TNMKEFVGDD IATEYSALMS KVVADGTLKV
     KFPINEPALA KKKSQIDEYL EFYGGAGVQH IALNTGDIVE TVRTMRAAGV QFLDTPDSYY
     DTLGEWVGDT RVPVDTLREL KILADRDEDG YLLQIFTKPV QDRPTVFFEI IERHGSMGFG
     KGNFKALFEA IEREQEKRGN L
//