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[1]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.01899-06; PubMed=17209016 [NCBI, ExPASy, EBI, Israel, Japan]
Lykidis A.,
Mavromatis K.,
Ivanova N.,
Anderson I.,
Land M.,
DiBartolo G.,
Martinez M.,
Lapidus A.,
Lucas S.,
Copeland A.,
Richardson P.,
Wilson D.B.,
Kyrpides N.;
"Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX.";
J. Bacteriol. 189:2477-2486(2007).
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[2]
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FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, SUBUNIT, AND BIOPHYSICOCHEMICAL PROPERTIES.
DOI=10.1007/s00253-004-1749-5; PubMed=15599520 [NCBI, ExPASy, EBI, Israel, Japan]
Fraaije M.W.,
Wu J.,
Heuts D.P.H.M.,
van Hellemond E.W.,
Lutje Spelberg J.H.,
Janssen D.B.;
"Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining.";
Appl. Microbiol. Biotechnol. 66:393-400(2005).
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[3]
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X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH FAD.
DOI=10.1073/pnas.0404538101; PubMed=15328411 [NCBI, ExPASy, EBI, Israel, Japan]
Malito E.,
Alfieri A.,
Fraaije M.W.,
Mattevi A.;
"Crystal structure of a Baeyer-Villiger monooxygenase.";
Proc. Natl. Acad. Sci. U.S.A. 101:13157-13162(2004).
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- FUNCTION: Catalyzes a Baeyer-Villiger oxidation reaction, i.e., the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Is most efficient with phenylacetone as substrate, leading to the formation of benzyl acetate. Can also oxidize other aromatic ketones (benzylacetone, alpha-methylphenylacetone and 4-hydroxyacetophenone), some alipatic ketones (dodecan-2-one and bicyclohept-2-en-6-one) and sulfides (e.g. methyl 4-tolylsulfide).
- CATALYTIC ACTIVITY: Phenylacetone + NADPH + O2 = benzyl acetate + NADP+ + H2O.
- COFACTOR: Binds 1 FAD per subunit.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=3 µM for NADPH; | | KM=59 µM for phenylacetone; | | KM=360 µM for benzylacetone; | | KM=830 µM for alpha-methylphenylacetone; | | KM=2.2 mM for 4-hydroxyacetophenone; | | KM=260 µM for 2-dodecanone; | | KM=15 mM for bicyclohept-2-en-6-one; | | KM=860 µM for methyl 4-tolylsulfide; | | pH dependence: |
Optimum pH is 8.0; | | Temperature dependence: |
Thermostable. Displays an activity half-life of 1 day at 52 degrees Celsius; | |
- SUBUNIT: Monomer.
- SIMILARITY: Belongs to the FAD-binding monooxygenase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 542 AA [This is the length of the unprocessed precursor] |
Molecular weight: 61124 Da [This is the MW of the unprocessed precursor] |
CRC64: B24DF347F3D23D02 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MAGQTTVDSR RQPPEEVDVL VVGAGFSGLY ALYRLRELGR SVHVIETAGD VGGVWYWNRY
70 80 90 100 110 120
PGARCDIESI EYCYSFSEEV LQEWNWTERY ASQPEILRYI NFVADKFDLR SGITFHTTVT
130 140 150 160 170 180
AAAFDEATNT WTVDTNHGDR IRARYLIMAS GQLSVPQLPN FPGLKDFAGN LYHTGNWPHE
190 200 210 220 230 240
PVDFSGQRVG VIGTGSSGIQ VSPQIAKQAA ELFVFQRTPH FAVPARNAPL DPEFLADLKK
250 260 270 280 290 300
RYAEFREESR NTPGGTHRYQ GPKSALEVSD EELVETLERY WQEGGPDILA AYRDILRDRD
310 320 330 340 350 360
ANERVAEFIR NKIRNTVRDP EVAERLVPKG YPFGTKRLIL EIDYYEMFNR DNVHLVDTLS
370 380 390 400 410 420
APIETITPRG VRTSEREYEL DSLVLATGFD ALTGALFKID IRGVGNVALK EKWAAGPRTY
430 440 450 460 470 480
LGLSTAGFPN LFFIAGPGSP SALSNMLVSI EQHVEWVTDH IAYMFKNGLT RSEAVLEKED
490 500 510 520 530 540
EWVEHVNEIA DETLYPMTAS WYTGANVPGK PRVFMLYVGG FHRYRQICDE VAAKGYEGFV
LT
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Q47PU3 in FASTA format |
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