NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P.;
"Complete sequence of Dechloromonas aromatica RCB.";
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000089; AAZ47082.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_285552.1; -.

3D structure databases

ModBase

Q47DJ9.

Enzyme and pathway databases

BioCyc

DARO159087:DARO_2346-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0033739;	Molecular function: queuine synthase activity (inferred from electronic annotation from EC).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

Q47DJ9.

Genome annotation databases

GeneID

3566066; -.

GenomeReviews

CP000089_GR; Daro_2346.

KEGG

dar:Daro_2346; -.

NMPDR

fig|159087.4.peg.2323; -.

Phylogenomic databases

HOGENOM

Q47DJ9; -.

Genome annotation databases

CMR

Q47DJ9; Daro_2346.

Other

ProtoNet

Q47DJ9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 283`	`283`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000247708`

Sequence information

Length: 283 AA [This is the length of the unprocessed precursor]

Molecular weight: 31427 Da [This is the MW of the unprocessed precursor]

CRC64: 4520F9606FAB8E03 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTLTTTHIAD PSHASPLGKA TEYQSHYAPE LLYPIPRQLK RSELGITDAN LPFVGEDLWN 

        70         80         90        100        110        120 
AYELSWLNSK GKPVVAVGTF RVPANSPNLI ESKSFKLYLN SFNQSSFDNI AAVSATMSRD 

       130        140        150        160        170        180 
LSAAAGSPII VTLEPLSASP LASIGTPDGI LLDELDITCD RYQPEPALLA TLPGENVEET 

       190        200        210        220        230        240 
LYSHLLKSNC LVTGQPDWAM VVIRYRGKPI DRAGLLRYIV SFRNHNEFHE QCVERIFSDI 

       250        260        270        280 
RVRCQPEVLA VHARYTRRGG LDINPFRSTG DYAAPDNTRE IRQ

Q47DJ9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools