NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
STRAIN=ES4;
PubMed=8349661 [NCBI, ExPASy, EBI, Israel, Japan]
Diruggiero J., Robb F.T., Jagus R., Klump H.H., Borges K.M., Kessel M., Mai X., Adams M.W.W.A.;
"Characterization, cloning, and in vitro expression of the extremely thermostable glutamate dehydrogenase from the hyperthermophilic Archaeon, ES4.";
J. Biol. Chem. 268:17767-17774(1993).

Comments

CATALYTIC ACTIVITY: L-glutamate + H₂O + NAD(P)⁺ = 2-oxoglutarate + NH₃ + NAD(P)H.
SUBUNIT: Homohexamer.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L12408; AAA64795.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A47410; A47410.

3D structure databases

HSSP

P80319; 1GTM. [HSSP ENTRY / PDB]

SMR

Q47951; 5-420.

ModBase

Q47951.

Ontologies

GO:0004353;	Molecular function: glutamate dehydrogenase [NAD(P)+] activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11606:SF2; GLFV_DH; 1.

Pfam

PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000185; Glu_DH; 1.

PRINTS

PR00082; GLFDHDRGNASE.

PROSITE

PS00074; GLFV_DEHYDROGENASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; NAD; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 420`	`420`	`Glutamate dehydrogenase.`	`PRO_0000182756`
`NP_BIND`	`220 226`	`7`	`NAD (Potential).`
`ACT_SITE`	`105 105`		`By similarity.`

Sequence information

Length: 420 AA [This is the length of the unprocessed precursor]

Molecular weight: 47141 Da [This is the MW of the unprocessed precursor]

CRC64: 26C571CC5DEF85CB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVEQDPFEIA VKQLERAAQY MKISEEALEF LKRPQRIVEV TIPVEMDDGT VKVFTGFRVQ 

        70         80         90        100        110        120 
YNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP YGGGKGGIIV DPKKLSDREK 

       130        140        150        160        170        180 
ERLARGYIRA IYDVISPYED IPAPDVYTNP QIMAWMMDEY EAISRRKTPA FGIITGKPLS 

       190        200        210        220        230        240 
IGGSLGRNEA TARGASYTIR EARKVLGWGD LKGKTIAIQG YGNAGYYLAK IMSEDYGMKV 

       250        260        270        280        290        300 
VAVSDSKGGI YNPDGLNADE VLKWKQEHGS VKDFPGATNI TNEELLELEV DVLAPAAIEE 

       310        320        330        340        350        360 
VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT VSYFEWVQNI 

       370        380        390        400        410        420 
TGYYWTLEEV REKLDKKMTK AFYDVYNTAK EKNIHMRDAD YVVAVQRVYQ AMLDRGWVKH

Q47951 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools