ID   PYRDB_ENTFA             Reviewed;         312 AA.
AC   Q47741;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 2.
DT   25-NOV-2008, entry version 69.
DE   RecName: Full=Dihydroorotate dehydrogenase B, catalytic subunit;
DE            EC=1.3.3.1;
DE   AltName: Full=Dihydroorotate oxidase B;
DE   AltName: Full=DHOdehase B;
DE            Short=DHODase B;
DE            Short=DHOD B;
GN   Name=pyrDB; Synonyms=pyrD, pyrD-2; OrderedLocusNames=EF_1714;
OS   Enterococcus faecalis (Streptococcus faecalis).
OC   Bacteria; Firmicutes; Lactobacillales; Enterococcaceae; Enterococcus.
OX   NCBI_TaxID=1351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 47077 / OG1RF;
RX   MEDLINE=96074317; PubMed=7592480;
RA   Li X., Weinstock G.M., Murray B.E.;
RT   "Generation of auxotrophic mutants of Enterococcus faecalis.";
RL   J. Bacteriol. 177:6866-6873(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=V583 / ATCC 700802;
RX   MEDLINE=22550857; PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F.,
RA   Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J.,
RA   Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A.,
RA   Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant
RT   Enterococcus faecalis.";
RL   Science 299:2071-2074(2003).
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=ATCC 29212 / DSM 2570;
RX   MEDLINE=99459259; PubMed=10529184; DOI=10.1021/bi990674q;
RA   Marcinkeviciene J., Tinney L.M., Wang K.H., Rogers M.J.,
RA   Copeland R.A.;
RT   "Dihydroorotate dehydrogenase B of Enterococcus faecalis.
RT   Characterization and insights into chemical mechanism.";
RL   Biochemistry 38:13129-13137(1999).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + O(2) = orotate +
CC       H(2)O(2).
CC   -!- COFACTOR: Binds 1 FMN per subunit.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 4/6.
CC   -!- SUBUNIT: Heterotetramer of 2 pyrK and 2 pyrD subunits (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the dihydroorotate dehydrogenase family.
CC       Type 1 subfamily.
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DR   EMBL; U24692; AAA67066.1; -; Genomic_DNA.
DR   EMBL; AE016830; AAO81490.1; -; Genomic_DNA.
DR   RefSeq; NP_815420.1; -.
DR   HSSP; P54322; 1EP2.
DR   SMR; Q47741; 4-309.
DR   GeneID; 1200606; -.
DR   GenomeReviews; AE016830_GR; EF_1714.
DR   KEGG; efa:EF1714; -.
DR   NMPDR; fig|226185.1.peg.1605; -.
DR   TIGR; EF_1714; -.
DR   HOGENOM; Q47741; -.
DR   BioCyc; EFAE226185:EF_1714-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0004158; F:dihydroorotate oxidase activity; IEA:HAMAP.
DR   GO; GO:0006207; P:'de novo' pyrimidine base biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006222; P:UMP biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_00224; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR012135; DHO_DHase_1_2.
DR   InterPro; IPR005720; DHO_DHase_1_core.
DR   InterPro; IPR001295; Dihydroorotate_DHase_core.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01180; DHO_dh; 1.
DR   PIRSF; PIRSF000164; DHO_oxidase; 1.
DR   TIGRFAMs; TIGR01037; pyrD_sub1_fam; 1.
DR   PROSITE; PS00911; DHODEHASE_1; 1.
DR   PROSITE; PS00912; DHODEHASE_2; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Flavoprotein; FMN; Oxidoreductase;
KW   Pyrimidine biosynthesis.
FT   CHAIN         1    312       Dihydroorotate dehydrogenase B, catalytic
FT                                subunit.
FT                                /FTId=PRO_0000148391.
FT   ACT_SITE    133    133       Nucleophile (By similarity).
FT   CONFLICT     81     81       E -> D (in Ref. 1; AAA67066).
SQ   SEQUENCE   312 AA;  33093 MW;  B97956521B85541F CRC64;
     MMKNPLAVSI PGLTLKNPII PASGCFGFGE EYANYYDLDQ LGSIMIKATT PQARYGNPTP
     RVAETPSGML NAIGLQNPGL EVVMQEKLPK LEKYPNLPII ANVAGACEED YVAVCAKIGQ
     APNVKAIELN ISCPNVKHGG IAFGTDPEVA FQLTQAVKKV ASVPIYVKLS PNVTDIVPIA
     QAIEAGGADG FSMINTLLGM RIDLKTRKPI LANQTGGLSG PAIKPVAIRL IRQVASVSQL
     PIIGMGGVQT VDDVLEMFMA GASAVGVGTA NFTDPYICPK LIDGLPKRME ELGIESLEQL
     IKEVREGQQN AR
//