NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.;
"Complete sequence of chromosome 2 of Ralstonia eutropha JMP134.";
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate (By similarity).
CATALYTIC ACTIVITY: 3-hydroxyanthranilate + O₂ = 2-amino-3-carboxymuconate semialdehyde.
COFACTOR: Binds 2 Fe(2+) ions per subunit (By similarity).
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the 3-HAO family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000091; AAZ64847.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_299691.1; -.

3D structure databases

SMR

Q46PT7; 4-175.

ModBase

Q46PT7.

Enzyme and pathway databases

BioCyc

REUT264198:REUT_B5502-MON; -.

Ontologies

GO:0000334;	Molecular function: 3-hydroxyanthranilate 3,4-dioxygenase activity (inferred from electronic annotation from HAMAP).
GO:0008198;	Molecular function: ferrous iron binding (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00825; -; 1.
PBIL [Tree]

InterPro

IPR010329; 3hydroanth_dOase.
Graphical view of domain structure.

Pfam

PF06052; 3-HAO; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR03037; anthran_nbaC; 1.

BLOCKS

Q46PT7.

Genome annotation databases

GeneID

3614547; -.

GenomeReviews

CP000091_GR; Reut_B5502.

KEGG

reu:Reut_B5502; -.

NMPDR

fig|264198.3.peg.6202; -.

Phylogenomic databases

HOGENOM

Q46PT7; -.

Genome annotation databases

CMR

Q46PT7; Reut_B5502.

Other

ProtoNet

Q46PT7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 189`	`189`	`3-hydroxyanthranilate 3,4-dioxygenase.`	`PRO_0000245476`
`METAL`	`53 53`		`Iron 1 (catalytic) (By similarity).`
`METAL`	`59 59`		`Iron 1 (catalytic) (By similarity).`
`METAL`	`97 97`		`Iron 1 (catalytic) (By similarity).`
`METAL`	`127 127`		`Iron 2 (By similarity).`
`METAL`	`130 130`		`Iron 2 (By similarity).`
`METAL`	`165 165`		`Iron 2 (By similarity).`
`METAL`	`168 168`		`Iron 2 (By similarity).`
`BINDING`	`49 49`		`Dioxygen (By similarity).`
`BINDING`	`59 59`		`Substrate (By similarity).`
`BINDING`	`101 101`		`Substrate (By similarity).`
`BINDING`	`112 112`		`Substrate (By similarity).`

Sequence information

Length: 189 AA [This is the length of the unprocessed precursor]

Molecular weight: 21938 Da [This is the MW of the unprocessed precursor]

CRC64: F3EE0A8DCEEEB74E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFDLKYGRPL NFKRWLDDHA DKLKPPVGNQ QIWQDSDMMV TVVGGPNERS DFHDDPIEEL 

        70         80         90        100        110        120 
FYQFKGNAYL LLWEDGRYER VDLREGDMLL LPPHTLHSPQ RPEADSRCLV VERKRPQGQN 

       130        140        150        160        170        180 
DAFQWSCASC GTIVQRHEVT LQSIVADLPP LYEKFYASSE DARRCPGCGE IHPGRDFQAW 


HRTLARHSS

Q46PT7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools