[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=ATCC 51845 / DSM 6160 / DES11 / LMG 18890; Thurner C.A.K.; Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: A primary alcohol + acceptor = an aldehyde + reduced acceptor.
COFACTOR: Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-143-Cys-144 and the indole ring of Trp-281 (By similarity).
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
COFACTOR: Binds 1 heme group per subunit (By similarity).
SUBUNIT: Heterotetramer (dehydrogenase, cytochrome, and two smaller unknown subunits) that forms the alcohol dehydrogenase complex (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Periplasmic side (Potential).
SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family.
SIMILARITY: Contains 1 cytochrome c domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X82894; CAA58066.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y09480; CAA70688.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

Q46444; 1KB0. [HSSP ENTRY / PDB]

ModBase

Q44002.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0018468;	Molecular function: alcohol dehydrogenase (acceptor) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR009056; Cyt_c_monohaem.
IPR017512; PQQ_MeOH/EtOH_DH.
IPR002372; PQQ_repeat.
IPR011047; Quino_AlcDHase-like.
IPR001479; Quinoprotein_DHase_PQQ_bac_CS.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.760.10; Cytochrome_c_R; 1.
G3DSA:2.140.10.10; Quinoprotein_alc_DH-like; 1.

Pfam

PF01011; PQQ; 6.
Pfam graphical view of domain structure.

SMART

SM00564; PQQ; 5.
SMART graphical view of domain structure.

PROSITE

PS00363; BACTERIAL_PQQ_1; 1.
PS00364; BACTERIAL_PQQ_2; 1.
PS51007; CYTC; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Cell membrane; Heme; Iron; Membrane; Metal-binding; Oxidoreductase; Periplasm; PQQ; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 35`	`35`	`Potential.`
`CHAIN`	`36 739`	`704`	`Alcohol dehydrogenase [acceptor].`	`PRO_0000025560`
`DOMAIN`	`635 739`	`105`	`Cytochrome c.`
`ACT_SITE`	`344 344`		`Proton acceptor (Potential).`
`METAL`	`217 217`		`Calcium (By similarity).`
`METAL`	`299 299`		`Calcium (By similarity).`
`METAL`	`655 655`		`Iron (heme axial ligand) (By similarity).`
`BINDING`	`651 651`		`Heme (covalent) (By similarity).`
`BINDING`	`654 654`		`Heme (covalent) (By similarity).`
`DISULFID`	`143 144`		`By similarity.`

Sequence information

Length: 739 AA [This is the length of the unprocessed precursor]

Molecular weight: 80945 Da [This is the MW of the unprocessed precursor]

CRC64: E681BB237ACB91F4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MISAVFGKRR SLSRTLTAGT ICAALISGYA TMASADDGQG ATGEAIIHAD DHPGNWMTYG 

        70         80         90        100        110        120 
RTYSEQRYSP LDQINRSNVG NLKLAWYLDL DTNRGQEGTP LVIDGVMYAT TNWSMMKAVD 

       130        140        150        160        170        180 
AATGKLLWSY DPRVPGNIAD KGCCDTVNRG AAYWNGKVYF GTFDGRLIAL DAKTGKLVWS 

       190        200        210        220        230        240 
VNTIPPEAEL GKQRSYTVDG APRIAKGRVI IGNGGSEFGA RGFVTAFDAE TGKVDWRFFT 

       250        260        270        280        290        300 
APNPKNEPDH TASDSVLMNK AYQTWSPTGA WTRQGGGGTV WDSIVYDPVA DLVYLGVGNG 

       310        320        330        340        350        360 
SPWNYKYRSE GKGDNLFLGS IVALKPETGE YVWHFQETPM DQWDFTSVQQ IMTLDLPING 

       370        380        390        400        410        420 
ETRHVIVHAP KNGFFYIIDA KTGEFISGKN YVYVNWASGL DPKTGRPIYN PDALYTLTGK 

       430        440        450        460        470        480 
EWYGIPGDLG GHNFAAMAFS PKTGLVYIPA QQVPFLYTNQ VGGFTPHPDS WNLGLDMNKV 

       490        500        510        520        530        540 
GIPDSPEAKQ AFVKDLKGWI VAWDPQKQAE AWRVDHKGPW NGGILATGGD LLFQGLANGE 

       550        560        570        580        590        600 
FHAYDATNGS DLFHFAADSG IIAPPVTYLA NGKQYVAVEV GWGGIYPFFL GGLARTSGWT 

       610        620        630        640        650        660 
VNHSRIIAFS LDGKSGPLPK QNDQGFLPVK PPAQFDSKRT DNGYFQFQTY CAACHGDNAE 

       670        680        690        700        710        720 
GAGVLPDLRW SGSIRHEDAF YNVVGRGALT AYGMDRFDGN MNPTEIEDIR QFLIKRANET 

       730 
YQREVDARKN ADGIPEQLP

Q44002 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools