[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Samsun; TISSUE=Leaf; Knight J.S., Emes M.J.; "Isolation of a tobacco chloroplast glucose-6-phosphate dehydrogenase cDNA."; (er) Plant Gene Register PGR96-076.

Comments

FUNCTION: Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division (By similarity).
CATALYTIC ACTIVITY: D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH.
ENZYME REGULATION: Regulated by metabolites. Post-translationally inactivated by cysteine-mediated redox modification via the ferredoxin-thioredoxin system in the light and this avoids futile cycles with photosynthetic CO2 fixation (By similarity).
PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast.
SIMILARITY: Belongs to the glucose-6-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X99405; CAA67782.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T03244; T03244.

3D structure databases

HSSP

P11413; 1QKI. [HSSP ENTRY / PDB]

ModBase

Q43793.

Family and domain databases

InterPro

IPR001282; Glc-6-P_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR23429; G6PDH; 1.

Pfam

PF02781; G6PD_C; 1.
PF00479; G6PD_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000110; G6PD; 1.

PRINTS

PR00079; G6PDHDRGNASE.

ProDom

PD001129; G6PD; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR00871; zwf; 1.

PROSITE

PS00069; G6P_DEHYDROGENASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Carbohydrate metabolism; Chloroplast; Glucose metabolism; NADP; Oxidoreductase; Plastid; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Chloroplast (Potential).`
`CHAIN`	`? 593`		`Glucose-6-phosphate 1-dehydrogenase, chloroplastic.`	`PRO_0000010441`
`ACT_SITE`	`345 345`		`Proton acceptor (By similarity).`
`BINDING`	`118 118`		`NADP (By similarity).`
`BINDING`	`150 150`		`NADP (By similarity).`
`BINDING`	`283 283`		`Substrate (By similarity).`
`BINDING`	`287 287`		`Substrate (By similarity).`
`DISULFID`	`168 176`		`Redox modulation (By similarity).`

Sequence information

Length: 593 AA [This is the length of the unprocessed precursor]

Molecular weight: 67369 Da [This is the MW of the unprocessed precursor]

CRC64: 228A7732E510E571 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVTLYSSPST HSSGPVASYS NSSIGLYNYH HNKQIAVSSI LSRKFGSLQI NQKPFWNAVR 

        70         80         90        100        110        120 
MQDGAVATPP SKIENETPLK KLKNGILPVA PPKEQKDTID FDSNKAKSTV SITVVGASGD 

       130        140        150        160        170        180 
LAKKKIFPAL FALYYEGCLP EHFTIFGYAR SKMTDAELRN MVSKTLTCRI DKRENCGEKM 

       190        200        210        220        230        240 
EQFLERCFYH SGQYDSLENF AELDKKLKEH EAGRFSNRLF YLSIPPNIFI NAVRCASLSA 

       250        260        270        280        290        300 
SSAHGWTRVI VEKPFGRDSE SSAALTRSLK QYLNEDQIFR IDHYLGKELV ENLSVLRFSN 

       310        320        330        340        350        360 
LIFEPLWSRQ CIRNVQFIFS EDFGTEGRGG YFDHYGIIRD IMQNHLLQIL ALFAMETPVS 

       370        380        390        400        410        420 
LDAEDIRNEK VKVLRSMRPL QLDDVIIGQY KCHTKGDVTY PGYTDDKTVP KDSLTPTFAA 

       430        440        450        460        470        480 
AALFIDNARW DGVPFLMKAG KALHTRSAEI RVQFRHVPGN LYNKNFGSDL DQATNELVIR 

       490        500        510        520        530        540 
VQPNEAIYLK INNKVPGLGM RLDRSNLNLL YSARYSKEIP DAYERLLLDA IEGERRLFIR 

       550        560        570        580        590 
SDELDAAWSL FTPVLKELED KKIVPEYYPY GSRGPIGAHY LAARYKVRWG DLV

Q43793 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools