NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
STRAIN=cv. Black forest provenance;
DOI=10.1007/BF00043873; PubMed=8049370 [NCBI, ExPASy, EBI, Israel, Japan]
Neininger A., Seith B., Hoch B., Mohr H.;
"Gene expression of nitrite reductase in Scots pine (Pinus sylvestris L.) as affected by light and nitrate.";
Plant Mol. Biol. 25:449-457(1994).

Comments

CATALYTIC ACTIVITY: NH₃ + 2 H₂O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H⁺.
COFACTOR: Binds 1 siroheme per subunit (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast (By similarity).
TISSUE SPECIFICITY: Highest expression in roots and hypocotyls. Some expression in cotyledonary whorls.
INDUCTION: Strong induction by nitrate in roots and hypocotyls. Strong induction by light and weak induction by nitrate in cotyledonary whorls.
SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X74949; CAA52905.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S46311; S46311.

3D structure databases

SMR

Q43090; 1-100.

ModBase

Q43090.

Family and domain databases

InterPro

IPR006066; Nir_Si_BS.
Graphical view of domain structure.

PS00365; NIR_SIR; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Chloroplast; Heme; Iron; Iron-sulfur; Metal-binding; Nitrate assimilation; Oxidoreductase; Plastid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 105`	`>105`	`Ferredoxin--nitrite reductase, chloroplastic.`	`PRO_0000199955`
`METAL`	`28 28`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`32 32`		`Iron (siroheme axial ligand) (By similarity).`
`METAL`	`32 32`		`Iron-sulfur (4Fe-4S) (By similarity).`
`NON_TER`	`1 1`

Sequence information

Length: 105 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 11597 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 1A9E45BF8846017E [This is a checksum on the sequence]

        10         20         30         40         50         60 
KARALKITEE LDRTMEVPKP VRMHWTGCPN TCAQVQVADI GFMGCMTRDE NKKVVEGVDI 

        70         80         90        100 
FIGGRVGADS HLGDLIHKGV PCKDVVPVVQ ELLIKHFGAI RKTNM

Q43090 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools