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UniProtKB/Swiss-Prot entry Q3T001

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name H17B6_BOVIN
Primary accession number Q3T001
Secondary accession numbers None
Integrated into Swiss-Prot on September 11, 2007
Sequence was last modified on October 11, 2005 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 24)
Name and origin of the protein
Protein name Hydroxysteroid 17-beta dehydrogenase 6 [Precursor]
Synonyms EC 1.1.1.62
EC 1.1.1.63
EC 1.1.1.105
17-beta-HSD6
Oxidative 3-alpha hydroxysteroid dehydrogenase
3-alpha->beta-hydroxysteroid epimerase
3-alpha->beta-HSE
Gene name
Name: HSD17B6
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Hereford;
TISSUE=Testis;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
Comments
  • FUNCTION: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids (By similarity).
  • CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
  • CATALYTIC ACTIVITY: Testosterone + NAD+ = androst-4-ene-3,17-dione + NADH.
  • CATALYTIC ACTIVITY: Retinol + NAD+ = retinal + NADH.
  • SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein; Lumenal side (By similarity). Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side (By similarity).
  • SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BC102626; AAI02627.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001030472.1; -.
UniGene Bt.16385
3D structure databases
ModBase Q3T001.
Ontologies
GO
GO:0004303; Molecular function: estradiol 17-beta-dehydrogenase activity (inferred from electronic annotation from EC).
GO:0004745; Molecular function: retinol dehydrogenase activity (inferred from electronic annotation from EC).
GO:0050327; Molecular function: testosterone 17-beta-dehydrogenase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR19410; ADH_short_C2; 1.
Pfam PF00106; adh_short; 1.
Pfam graphical view of domain structure.
PRINTS PR00081; GDHRDH.
PR00080; SDRFAMILY.
PROSITE PS00061; ADH_SHORT; 1.
BLOCKS Q3T001.
Genome annotation databases
Ensembl ENSBTAG00000011720; Bos taurus. [Contig view]
GeneID 533086; -.
KEGG bta:533086; -.
Phylogenomic databases
HOVERGEN Q3T001; -.
Other
ProtoNet Q3T001.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Endoplasmic reticulum; Glycoprotein; Lipid metabolism; Membrane; Microsome; NAD; Oxidoreductase; Signal; Steroid metabolism.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    17  17     Potential. 
CHAIN   18   317  300     Hydroxysteroid 17-beta dehydrogenase 6. PRO_0000303210
NP_BIND   33    57  25     NAD (By similarity). 
ACT_SITE   176   176        Proton acceptor (By similarity). 
BINDING   164   164        Substrate (Potential). 
CARBOHYD   71    71        N-linked (GlcNAc...) (Potential). 
CARBOHYD   161   161        N-linked (GlcNAc...) (Potential). 
Sequence information
Length: 317 AA [This is the length of the unprocessed precursor] Molecular weight: 36198 Da [This is the MW of the unprocessed precursor] CRC64: 4B1A2ED7906FBB12 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MWLYLAVLLG LYYLLRWFRE RQVVSHLQDK FVFITGCDSG FGNQLARQLD LRGLRVLAGC 

        70         80         90        100        110        120 
LTEQGAEQLR NQTSDRLQTV ILDVTKTESI AAATEWVKEC VGDRGLWGLV NNAGIFHSHG 

       130        140        150        160        170        180 
YAEWIKIETY RDTLRVNLIG VIEVTLSMLP LVRKAQGRIV NVSSILGRIA FFGAVYSCSK 

       190        200        210        220        230        240 
YGVEAFSDIL RRELQHFGVK VSMVEPGYFR TAMTDWQKFS EIMKQIWKET PAHIKETYGQ 

       250        260        270        280        290        300 
KFFDAYHDLM KQGLLSCSTN LNLVTDCMEH ALTSVHPRTR YSAGWDAQFF FVPLSYLPTS 

       310 
LADYILTRSW PKPAQAV
Q3T001 in FASTA format

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