[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Crossbred X Angus; TISSUE=Ileum; NIH - Mammalian Gene Collection (MGC) project; Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5 (By similarity).
CATALYTIC ACTIVITY: NADPH + n oxidized hemoprotein = NADP⁺ + n reduced hemoprotein.
COFACTOR: FAD (By similarity).
COFACTOR: FMN (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Anchored to the ER membrane by its N-terminal hydrophobic region (By similarity).
SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC103399; AAI03400.1; ALT_INIT; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001030467.1; -.

UniGene

Bt.41220

3D structure databases

SMR

Q3SYT8; 64-678.

ModBase

Q3SYT8.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR015702; NADPH_Cyt_Red.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

PANTHER

PTHR19384:SF17; NADPH_Cyt_Red; 1.

Pfam

PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00369; FLAVODOXIN.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q3SYT8.

Genome annotation databases

Ensembl

ENSBTAG00000017082; Bos taurus. [Contig view]

GeneID

532512; -.

KEGG

bta:532512; -.

Phylogenomic databases

HOVERGEN

Q3SYT8; -.

Other

ProtoNet

Q3SYT8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; FAD; Flavoprotein; FMN; Membrane; NADP; Oxidoreductase; Phosphoprotein.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 678`	`677`	`NADPH--cytochrome P450 reductase.`	`PRO_0000245573`
`DOMAIN`	`80 224`	`145`	`Flavodoxin-like.`
`DOMAIN`	`279 521`	`243`	`FAD-binding FR-type.`
`NP_BIND`	`170 201`	`32`	`FMN (By similarity).`
`NP_BIND`	`314 325`	`12`	`FAD (By similarity).`
`NP_BIND`	`451 461`	`11`	`FAD (By similarity).`
`NP_BIND`	`529 547`	`19`	`NADP (By similarity).`
`NP_BIND`	`624 640`	`17`	`NADP (By similarity).`
`MOD_RES`	`575 575`		`Phosphotyrosine (By similarity).`

Sequence information

Length: 678 AA [This is the length of the unprocessed precursor]

Molecular weight: 77021 Da [This is the MW of the unprocessed precursor]

CRC64: C4103BC020D8842C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MADSNMDAGT TTSEMVAEEV SLFSTTDVIL FSLIVGVMTY WFLFRKKKEE VPEFTKIQTT 

        70         80         90        100        110        120 
TSSVKDRSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLA 

       130        140        150        160        170        180 
DLSSLPEIEK ALAIFCMATY GEGDPTDNAQ DFYDWLQETD VDLSGVKYAV FALGNKTYEH 

       190        200        210        220        230        240 
FNAMGKYVDK RLEQLGAQRI FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES 

       250        260        270        280        290        300 
SIRQYELMVH TDMDMAKVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL 

       310        320        330        340        350        360 
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGA DLDIIMSLNN LDEESNKKHP 

       370        380        390        400        410        420 
FPCPTSYRTA LTYYLDITNP PRTNVLYELA QYASEPTEHE QLRKMASSSG EGKELYLRWV 

       430        440        450        460        470        480 
LEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVAVEYET 

       490        500        510        520        530        540 
KTGRINKGVA TSWLRAKEPA GENGGRALVP MYVRKSQFRL PFKATTPVIM VGPGTGVAPF 

       550        560        570        580        590        600 
IGFIQERAWL RQQGKEVGET LLYYGCRRSD EDYLYREELA GFHKDGALTQ LNVAFSREQP 

       610        620        630        640        650        660 
QKVYVQHLLK KDKEHLWKLI HEGGAHIYVC GDARNMARDV QNTFYDIVAE QGAMEHAQAV 

       670 
DYVKKLMTKG RYSLDVWS

Q3SYT8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools