ID   CP4F3_RAT               Reviewed;         524 AA.
AC   Q3MID2;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   04-NOV-2008, entry version 26.
DE   RecName: Full=Cytochrome P450 4F3;
DE            EC=1.14.13.30;
DE   AltName: Full=CYPIVF3;
DE   AltName: Full=Leukotriene-B(4) omega-hydroxylase;
DE   AltName: Full=Leukotriene-B(4) 20-monooxygenase;
DE   AltName: Full=Cytochrome P450-LTB-omega;
GN   Name=Cyp4f3; Synonyms=Cyp4f18;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Cytochromes P450 are a group of heme-thiolate
CC       monooxygenases. This enzyme requires molecular oxygen and NADPH
CC       for the omega-hydroxylation of LTB4, a potent chemoattractant for
CC       polymorphonuclear leukocytes (By similarity).
CC   -!- CATALYTIC ACTIVITY: (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-
CC       6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)-
CC       5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- ENZYME REGULATION: Inhibited by carbon monoxide (CO) (By
CC       similarity).
CC   -!- PATHWAY: Lipid metabolism; leukotriene B4 degradation.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein (By similarity). Microsome membrane; Single-pass
CC       membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BC101918; AAI01919.1; -; mRNA.
DR   RefSeq; NP_001028858.1; -.
DR   UniGene; Rn.215683; -.
DR   Ensembl; ENSRNOG00000015751; Rattus norvegicus.
DR   GeneID; 290623; -.
DR   KEGG; rno:290623; -.
DR   RGD; 1305261; Cyp4f18.
DR   HOVERGEN; Q3MID2; -.
DR   NextBio; 631306; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005792; C:microsome; IEA:UniProtKB-KW.
DR   GO; GO:0050051; F:leukotriene-B4 20-monooxygenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; NADP; Oxidoreductase; Transmembrane.
FT   CHAIN         1    524       Cytochrome P450 4F3.
FT                                /FTId=PRO_0000238925.
FT   TRANSMEM     15     35       Potential.
FT   METAL       468    468       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   524 AA;  59906 MW;  8DC9033E01F1A1E8 CRC64;
     MPLLSLSWLG LGHTAASPWL LLLLVGASCL LAYILPQVYA VFENSRRLRR FPQPPPRNWL
     FGHLGLIQSS EEGLLYIQSL SRTFRDVCCW WVGPWHPVIR IFHPAFIKPV ILAPASVAPK
     DRVFYRFLRP WLGDGLLLST GDKWSRHRRM LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR
     LASQGSARLD MFEHISLMTL DSLQKCVFSF DSNCQEKPSE YITAILELSA LVARRHQSLL
     LHVDLFYHLT RDGMRFRKAC RLVHDFTDAV IRERRCTLPD QGGDDALKAK AKAKTLDFID
     VLLLSKDEHG EALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLAKHPEY QERCRQEVRE
     LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPATAISR CCTQDIMLPD GRVIPKGVIC
     RISIFGTHHN PAVWPDPEVY NPFRFDADNG KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV
     ALALTLLRFR VLPDDKEPRR KPELILRAEG GLWLRVEPLS AGAH
//