NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes (By similarity).
CATALYTIC ACTIVITY: (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O₂ = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
ENZYME REGULATION: Inhibited by carbon monoxide (CO) (By similarity).
PATHWAY: Lipid metabolism; leukotriene B4 degradation .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Microsome membrane; Single-pass membrane protein (By similarity).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC101918; AAI01919.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001028858.1; -.

UniGene

Rn.215683

3D structure databases

ModBase

Q3MID2.

Organism-specific databases

RGD

1305261; Cyp4f18.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q3MID2.

Genome annotation databases

Ensembl

ENSRNOG00000015751; Rattus norvegicus. [Contig view]

GeneID

290623; -.

KEGG

rno:290623; -.

Phylogenomic databases

HOVERGEN

Q3MID2; -.

Other

ProtoNet

Q3MID2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 524`	`524`	`Cytochrome P450 4F3.`	`PRO_0000238925`
`TRANSMEM`	`15 35`	`21`	`Potential.`
`METAL`	`468 468`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 524 AA [This is the length of the unprocessed precursor]

Molecular weight: 59906 Da [This is the MW of the unprocessed precursor]

CRC64: 8DC9033E01F1A1E8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPLLSLSWLG LGHTAASPWL LLLLVGASCL LAYILPQVYA VFENSRRLRR FPQPPPRNWL 

        70         80         90        100        110        120 
FGHLGLIQSS EEGLLYIQSL SRTFRDVCCW WVGPWHPVIR IFHPAFIKPV ILAPASVAPK 

       130        140        150        160        170        180 
DRVFYRFLRP WLGDGLLLST GDKWSRHRRM LTPAFHFNIL KPYVKIFNDS TNIMHAKWQR 

       190        200        210        220        230        240 
LASQGSARLD MFEHISLMTL DSLQKCVFSF DSNCQEKPSE YITAILELSA LVARRHQSLL 

       250        260        270        280        290        300 
LHVDLFYHLT RDGMRFRKAC RLVHDFTDAV IRERRCTLPD QGGDDALKAK AKAKTLDFID 

       310        320        330        340        350        360 
VLLLSKDEHG EALSDEDIRA EADTFMFGGH DTTASGLSWI LYNLAKHPEY QERCRQEVRE 

       370        380        390        400        410        420 
LLRDREPEEI EWDDLAQLPF LTMCIKESLR LHPPATAISR CCTQDIMLPD GRVIPKGVIC 

       430        440        450        460        470        480 
RISIFGTHHN PAVWPDPEVY NPFRFDADNG KGRSPLAFIP FSAGPRNCIG QTFAMSEMKV 

       490        500        510        520 
ALALTLLRFR VLPDDKEPRR KPELILRAEG GLWLRVEPLS AGAH

Q3MID2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools