NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.;
"Complete sequence of Anabaena variabilis ATCC 29413.";
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4 .
PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4 .
SIMILARITY: Belongs to the ketol-acid reductoisomerase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000117; ABA19759.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_320654.1; -.

3D structure databases

ModBase

Q3MGX7.

Enzyme and pathway databases

BioCyc

AVAR240292:AVA_0133-MON; -.

Ontologies

GO:0004455;	Molecular function: ketol-acid reductoisomerase activity (inferred from electronic annotation from HAMAP).
GO:0009097;	Biological process: isoleucine biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0009099;	Biological process: valine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00435; -; 1.
PBIL [Tree]

InterPro

IPR013023; AcH_isomrdctse.
IPR000506; AcH_isomrdctse_C.
IPR013116; IlvN.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR21371; AcH_isomrdctse; 1.

Pfam

PF01450; IlvC; 1.
PF07991; IlvN; 1.
Pfam graphical view of domain structure.

TIGR00465; ilvC; 1.

Genome annotation databases

GeneID

3683350; -.

GenomeReviews

CP000117_GR; Ava_0133.

KEGG

ava:Ava_0133; -.

NMPDR

fig|240292.3.peg.526; -.

Phylogenomic databases

HOGENOM

Q3MGX7; -.

Genome annotation databases

CMR

Q3MGX7; Ava_0133.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 331`	`331`	`Ketol-acid reductoisomerase.`	`PRO_0000252749`
`ACT_SITE`	`108 108`		`Potential.`

Sequence information

Length: 331 AA [This is the length of the unprocessed precursor]

Molecular weight: 35997 Da [This is the MW of the unprocessed precursor]

CRC64: 3AF38044D6CE7F1C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MARMYYDEDA NLDLLAGKTI AIIGYGSQGH AHALNLKDSG LNVIVGLYPG SKSAEKAQAA 

        70         80         90        100        110        120 
GLTVKNVADA ANVADFIMIL LPDEVQKTVY KNEIEPNLQE GNTLAFAHGF NIHFGQVVPP 

       130        140        150        160        170        180 
ANVDVVMVAP KGPGHLVRRT YEQGQGVPAL FAVYQDASGK ARDRALSYAK GIGGTRGGVL 

       190        200        210        220        230        240 
ETTFREETET DLFGEQAVLC GGLSALIKAG FETLVEAGYQ PELAYFECLH EVKLIVDLVV 

       250        260        270        280        290        300 
EGGLAKMRDS ISNTAEYGDY TRGPRIVNEQ TKAEMRKVLS EIQSGQFARE FVLENQSGKP 

       310        320        330 
GFTAMRRQEA EHPIEEVGKD LRAMFSWLKK V

Q3MGX7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools