NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.;
"Complete sequence of Anabaena variabilis ATCC 29413.";
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000117; ABA24014.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_324909.1; -.

3D structure databases

ModBase

Q3M4S2.

Enzyme and pathway databases

BioCyc

AVAR240292:AVA_4416-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0033739;	Molecular function: queuine synthase activity (inferred from electronic annotation from EC).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

Q3M4S2.

Genome annotation databases

GeneID

3680446; -.

GenomeReviews

CP000117_GR; Ava_4416.

KEGG

ava:Ava_4416; -.

NMPDR

fig|240292.3.peg.4839; -.

Phylogenomic databases

HOGENOM

Q3M4S2; -.

Genome annotation databases

CMR

Q3M4S2; Ava_4416.

Other

ProtoNet

Q3M4S2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 136`	`136`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000247677`

Sequence information

Length: 136 AA [This is the length of the unprocessed precursor]

Molecular weight: 15548 Da [This is the MW of the unprocessed precursor]

CRC64: FC189826ACFB3D71 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSNSSPETVS QPSQEVKYGE REIAEGQLIT FPNPRVGRRY DINITLPEFT CKCPFSGYPD 

        70         80         90        100        110        120 
FATIYITYVP DERVVELKAL KLYINSYRDR YISHEESANQ ILDDFVAACD PLEATVKADF 

       130 
TPRGNVHTVV EVKHRK

Q3M4S2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools