NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
STRAIN=cv. Maris Huntsman;
TISSUE=Scutellum;
DOI=10.1007/s00425-005-0104-0; PubMed=16160850 [NCBI, ExPASy, EBI, Israel, Japan]
Appleford N.E., Evans D.J., Lenton J.R., Gaskin P., Croker S.J., Devos K.M., Phillips A.L., Hedden P.;
"Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat.";
Planta 223:568-582(2006).

Comments

FUNCTION: Converts the inactive gibbberellin precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Accepts also GA15, GA44, the 2,3-unsaturated GA5 and 2,3-dihydroGA9 as substrate. No activity with GA12, GA53, GA24, GA19 and GA25. Possesses also 2-beta-hydroxylase, 2,3-desaturase, 2,3-epoxidase and 13-hydroxylase activities.
CATALYTIC ACTIVITY: Gibberellin 20 + 2-oxoglutarate + O₂ = gibberellin 1 + succinate + CO₂.
COFACTOR: Ascorbate (By similarity).
COFACTOR: Iron (By similarity).
BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters: K_M=1 µM for GA9;
K_M=2.02 µM for GA20;
TISSUE SPECIFICITY: Expressed in internodes, nodes and the ear of the elongating stem.
DEVELOPMENTAL STAGE: Highly expressed in the embryo and the surrounding maternal tissues, the pericarp and the integuments. Also found in the germinating grain.
SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ118250; AAZ94377.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q3I411.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-11644; -.

Organism-specific databases

Gramene

Q3I411; -.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Iron; Metal-binding; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 370`	`370`	`Gibberellin 3-beta-dioxygenase 2-1.`	`PRO_0000067317`
`ACT_SITE`	`297 297`		`Potential.`
`METAL`	`229 229`		`Iron (By similarity).`
`METAL`	`231 231`		`Iron (By similarity).`
`METAL`	`287 287`		`Iron (By similarity).`

Sequence information

Length: 370 AA [This is the length of the unprocessed precursor]

Molecular weight: 40245 Da [This is the MW of the unprocessed precursor]

CRC64: 34930E368D91D3DD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPTPSHLSKD PRYFDFRAAR RVPETHAWPG LHDHPVVDGS GAGGGPDAVP VVDMRDPCAA 

        70         80         90        100        110        120 
EAVALAAQDW GAFLLEGHGV PLELLAGVEA AIGGMFALPA SEKMRAVRRP GDSCGYGSPP 

       130        140        150        160        170        180 
ISSFFSKCMW SEGYTFSPAN LRSDLRKLWP KAGHDYRHFC AVMEEFHREM RALADKLLEL 

       190        200        210        220        230        240 
FLVALGLTGE QVAAVESEHK IAETMTATMH LNWYPKCPDP KRALGLIAHT DSGFFTFVLQ 

       250        260        270        280        290        300 
SLVPGLQLFR HGPDRWVTVP AVPGAMVVNV GDLFQILTNG RFHSVYHRAV VNRDSDRISL 

       310        320        330        340        350        360 
GYFLGPPAHV KVAPLREALA GTPAAYRAVT WPEYMGVRKK AFTTGASALK MVAISTDNDA 

       370 
ANDTDDLISS

Q3I411 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools