NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.;
"Complete sequence of chromosome 1 of Burkholderia sp. 383.";
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000151; ABB09659.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_370303.1; -.

3D structure databases

ModBase

Q39D07.

Enzyme and pathway databases

BioCyc

BSP36773:BCEP18194_A6065-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

Q39D07.

Genome annotation databases

GeneID

3751297; -.

GenomeReviews

CP000151_GR; Bcep18194_A6065.

KEGG

bur:Bcep18194_A6065; -.

NMPDR

fig|269483.3.peg.7546; -.

Phylogenomic databases

HOGENOM

Q39D07; -.

Genome annotation databases

CMR

Q39D07; Bcep18194_A6065.

Other

ProtoNet

Q39D07.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 274`	`274`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000247705`

Sequence information

Length: 274 AA [This is the length of the unprocessed precursor]

Molecular weight: 30472 Da [This is the MW of the unprocessed precursor]

CRC64: 4563782498A6859F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNPEHSPLGK ATVYAAQYDA SLLFPIPRAG AREQLGITSA LPFFGTDIWN AYELSWLNAR 

        70         80         90        100        110        120 
GKPQIAVATF YVPAESPNIV ESKSFKLYLG SFAQTTFDSI DAVRDTLKRD VSAACGATVS 

       130        140        150        160        170        180 
VQLVSPHDFG KLEMEELDGL SLDRLDLDTD VYEPDPSLLK AAEDEAPVEE TLVSDLLRSN 

       190        200        210        220        230        240 
CPVTGQPDWG SVQIHYVGPQ IDHAGLLRYI ISFRNHTGFH EQCVERIFLD ILHACKPVKL 

       250        260        270 
AVYARYTRRG GLDINPFRTN YNQPMPDNAR NARQ

Q39D07 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools