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UniProtKB/Swiss-Prot entry Q39103


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name G3OX1_ARATH
Primary accession number Q39103
Secondary accession numbers None
Integrated into Swiss-Prot on December 6, 2005
Sequence was last modified on July 1, 1997 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 51)
Name and origin of the protein
Protein name Gibberellin 3-beta-dioxygenase 1
Synonyms EC 1.14.11.15
Gibberellin 3 beta-hydroxylase 1
GA 3-oxidase 1
AtGA3ox1
Gene name
Name: GA4
OrderedLocusNames: At1g15550
ORFNames: T16N11.6
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-220, INDUCTION, AND TISSUE SPECIFICITY.
STRAIN=cv. Landsberg erecta;
DOI=10.1105/tpc.7.2.195; PubMed=7756830 [NCBI, ExPASy, EBI, Israel, Japan]
Chiang H.-H., Hwang I., Goodman H.M.;
"Isolation of the Arabidopsis GA4 locus.";
Plant Cell 7:195-201(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
FUNCTION.
DOI=10.1104/pp.117.2.559; PubMed=9625708 [NCBI, ExPASy, EBI, Israel, Japan]
Williams J., Phillips A.L., Gaskin P., Hedden P.;
"Function and substrate specificity of the gibberellin 3beta-hydroxylase encoded by the Arabidopsis GA4 gene.";
Plant Physiol. 117:559-563(1998).
[5]
FUNCTION, INDUCTION, AND DEVELOPMENTAL STAGE.
DOI=10.1105/tpc.10.12.2115; PubMed=9836749 [NCBI, ExPASy, EBI, Israel, Japan]
Yamaguchi S., Smith M.W., Brown R.G.S., Kamiya Y., Sun T.-P.;
"Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds.";
Plant Cell 10:2115-2126(1998).
[6]
TISSUE SPECIFICITY.
PubMed=11737781 [NCBI, ExPASy, EBI, Israel, Japan]
Yamaguchi S., Kamiya Y., Sun T.-P.;
"Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination.";
Plant J. 28:443-453(2001).
[7]
CHARACTERIZATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
DOI=10.1104/pp.104.041509; PubMed=15181202 [NCBI, ExPASy, EBI, Israel, Japan]
Zhou R., Yu M., Pharis R.P.;
"16,17-dihydro gibberellin A5 competitively inhibits a recombinant Arabidopsis GA 3beta-hydroxylase encoded by the GA4 gene.";
Plant Physiol. 135:1000-1007(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L37126; AAC37506.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC013453; AAF71980.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT005827; AAO64762.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D86289; D86289.
RefSeq NP_173008.1; -.
UniGene At.10782
3D structure databases
ModBase Q39103.
Enzyme and pathway databases
BioCyc MetaCyc:AT1G15550-MON; -.
Organism-specific databases
GeneFarm 5167; 494.
TAIR At1g15550; -.
Gene expression databases
ArrayExpress Q39103; -.
GermOnline AT1G15550; Arabidopsis thaliana.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (traceable author statement from TAIR).
GO:0016707; Molecular function: gibberellin 3-beta-dioxygenase activity (traceable author statement from TAIR).
GO:0009740; Biological process: gibberellic acid mediated signaling (traceable author statement from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR005123; 2OG-FeII_Oase.
IPR002283; Isopenicillin-N_synthase.
Graphical view of domain structure.
Pfam PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.
PRINTS PR00682; IPNSYNTHASE.
BLOCKS Q39103.
Genome annotation databases
GeneID 838125; -.
GenomeReviews CT485782_GR; AT1G15550.
KEGG ath:AT1G15550; -.
NMPDR fig|3702.1.peg.1863; -.
Other
ProtoNet Q39103.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   358  358     Gibberellin 3-beta-dioxygenase 1. PRO_0000067313
ACT_SITE   299   299        Potential. 
METAL   232   232        Iron (By similarity). 
METAL   234   234        Iron (By similarity). 
METAL   289   289        Iron (By similarity). 
MUTAGEN   220   220        C->Y: In ga4-1; semidwarf and deficient in active gibberellins. 
Sequence information
Length: 358 AA [This is the length of the unprocessed precursor] Molecular weight: 40162 Da [This is the MW of the unprocessed precursor] CRC64: 26CED48F31554DEC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPAMLTDVFR GHPIHLPHSH IPDFTSLREL PDSYKWTPKD DLLFSAAPSP PATGENIPLI 

        70         80         90        100        110        120 
DLDHPDATNQ IGHACRTWGA FQISNHGVPL GLLQDIEFLT GSLFGLPVQR KLKSARSETG 

       130        140        150        160        170        180 
VSGYGVARIA SFFNKQMWSE GFTITGSPLN DFRKLWPQHH LNYCDIVEEY EEHMKKLASK 

       190        200        210        220        230        240 
LMWLALNSLG VSEEDIEWAS LSSDLNWAQA ALQLNHYPVC PEPDRAMGLA AHTDSTLLTI 

       250        260        270        280        290        300 
LYQNNTAGLQ VFRDDLGWVT VPPFPGSLVV NVGDLFHILS NGLFKSVLHR ARVNQTRARL 

       310        320        330        340        350 
SVAFLWGPQS DIKISPVPKL VSPVESPLYQ SVTWKEYLRT KATHFNKALS MIRNHREE 

Q39103 in FASTA format

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