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UniProtKB/Swiss-Prot entry Q38946


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHE2_ARATH
Primary accession number Q38946
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 2000
Sequence was last modified on November 1, 1996 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 67)
Name and origin of the protein
Protein name Glutamate dehydrogenase 2
Synonyms GDH 2
EC 1.4.1.3
Gene name
Name: GDH2
OrderedLocusNames: At5g07440
ORFNames: T2I1_150
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Turano F.J., Thakkar S.S., Weisemann J.M.;
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048507; PubMed=11130714 [NCBI, ExPASy, EBI, Israel, Japan]
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K., Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.;
"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
Nature 408:823-826(2000).
[3]
PROTEIN SEQUENCE OF 232-243 AND 391-399, AND SUBCELLULAR LOCATION.
TISSUE=Leaf, and Stem;
DOI=10.1104/pp.127.4.1694; PubMed=11743114 [NCBI, ExPASy, EBI, Israel, Japan]
Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P.;
"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis.";
Plant Physiol. 127:1694-1710(2001).
[4]
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1105/tpc.016055; PubMed=14671022 [NCBI, ExPASy, EBI, Israel, Japan]
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.;
"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.";
Plant Cell 16:241-256(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U56635; AAB01222.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL163912; CAB87933.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T49883; T49883.
RefSeq NP_001119183.1; -.
NP_196361.1; -.
UniGene At.21712
3D structure databases
HSSP P96110; 1B26. [HSSP ENTRY / PDB]
ModBase Q38946.
Organism-specific databases
GeneFarm 2247; 178.
TAIR At5g07440; -.
Gene expression databases
ArrayExpress Q38946; -.
GermOnline AT5G07440; Arabidopsis thaliana.
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11606:SF2; GLFV_DH; 1.
Pfam PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000185; Glu_DH; 1.
PRINTS PR00082; GLFDHDRGNASE.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS Q38946.
Proteomic databases
ProMEX Q38946; -.
Genome annotation databases
GeneID 830635; -.
GenomeReviews BA000015_GR; AT5G07440.
KEGG ath:AT5G07440; -.
NMPDR fig|3702.1.peg.22884; -.
Other
ProtoNet Q38946.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   411  411     Glutamate dehydrogenase 2. PRO_0000182746
ACT_SITE   102   102        By similarity. 
CONFLICT   232   232        V -> I (in Ref. 3; AA sequence). 
CONFLICT   242   243        IR -> LK (in Ref. 3; AA sequence). 
Sequence information
Length: 411 AA [This is the length of the unprocessed precursor] Molecular weight: 44699 Da [This is the MW of the unprocessed precursor] CRC64: 91267768215A15CF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNALAATNRN FRHASRILGL DSKIERSLMI PFREIKVECT IPKDDGTLVS YIGFRVQHDN 

        70         80         90        100        110        120 
ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVADIPYGG AKGGIGCSPR DLSLSELERL 

       130        140        150        160        170        180 
TRVFTQKIHD LIGIHTDVPA PDMGTNAQTM AWILDEYSKF HGHSPAVVTG KPIDLGGSLG 

       190        200        210        220        230        240 
REAATGRGVV FATEALLAEY GKSIQGLTFV IQGFGNVGTW AAKLIHEKGG KVVAVSDITG 

       250        260        270        280        290        300 
AIRNPEGIDI NALIKHKDAT GSLNDFNGGD AMNSDELLIH ECDVLIPCAL GGVLNKENAG 

       310        320        330        340        350        360 
DVKAKFIVEA ANHPTDPDAD EILSKKGVII LPDIYANAGG VTVSYFEWVQ NIQGFMWEEE 

       370        380        390        400        410 
KVNLELQKYM TRAFHNIKTM CHTHSCNLRM GAFTLGVNRV ARATQLRGWE A 

Q38946 in FASTA format

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