NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 30010 / Neff;
DOI=10.1006/jmbi.1994.0043; PubMed=7844823 [NCBI, ExPASy, EBI, Israel, Japan]
Burger G., Plante I., Lonergan K.M., Gray M.W.;
"The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii: complete sequence, gene content and genome organization.";
J. Mol. Biol. 245:522-537(1995).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
SUBUNIT: Complex I is composed of about 30 different subunits (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane (By similarity). Note=Matrix and cytoplasmic side of the mitochondrial inner membrane (By similarity).
SIMILARITY: Belongs to the complex I 75 kDa subunit family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U12386; AAD11824.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S53832; S53832.

RefSeq

NP_042531.1; -.

3D structure databases

ModBase

Q37373.

Family and domain databases

InterPro

IPR006058; 2Fe2S_fd_BS.
IPR001041; Ferredoxin.
IPR006656; Mopterin_OxRdtase.
IPR000283; NADH_DHase_75KDa_su_CS.
IPR010228; NADH_quinone_OxRdtase_G.
Graphical view of domain structure.

Pfam

PF00111; Fer2; 1.
PF00384; Molybdopterin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01973; NuoG; 1.

PROSITE

PS00197; 2FE2S_FER_1; FALSE_NEG.
PS51085; 2FE2S_FER_2; FALSE_NEG.
PS00641; COMPLEX1_75K_1; FALSE_NEG.
PS00642; COMPLEX1_75K_2; 1.
PS00643; COMPLEX1_75K_3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q37373.

Genome annotation databases

GeneID

1734026; -.

Other

ProtoNet

Q37373.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 4Fe-4S; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 675`	`675`	`NADH-ubiquinone oxidoreductase 75 kDa subunit.`	`PRO_0000118536`
`DOMAIN`	`2 80`	`79`	`2Fe-2S ferredoxin-type.`
`METAL`	`36 36`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`47 47`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`50 50`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`64 64`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`96 96`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`100 100`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`103 103`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`109 109`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`148 148`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`151 151`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`154 154`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`198 198`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`

Sequence information

Length: 675 AA [This is the length of the unprocessed precursor]

Molecular weight: 77554 Da [This is the MW of the unprocessed precursor]

CRC64: 3870F3BC9E8B9807 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKNISFKVND FQYTINNKLT LIQACLKNKV DISRFCFHEK LSIAGNCRMC LVEDLKQVKP 

        70         80         90        100        110        120 
LASCAINVSN SMNIYTNTLK VKKARESVLE FLLANHPLDC PICDQGGECD LQDQSVVFGS 

       130        140        150        160        170        180 
DRGRFYEFKR SVEDKDCGPL IKTIMNRCIH CTRCVRFSNE VAGVNILGVT GRGSKMEIGF 

       190        200        210        220        230        240 
YIENLMRSEL SGNVIDLCPV GALTSKPFAF TSRPWELKSY NSIDVLDSLH SNIRVDIRGT 

       250        260        270        280        290        300 
KIMRILPRVN SELNEDWITD KIRFSYDSFR RQRLYDPMVK ISGSFLKIGW KKAMLFIKKF 

       310        320        330        340        350        360 
FCNFLGFNHS SFIPLRGYIG DYLDLETIYT FKKFLLLNGS NFFLPSSSYN DLTALYSFNT 

       370        380        390        400        410        420 
PLTRLDEGDF CILLDVNLRV ELPIVNSRIK QLVSKKMLPV FVLGFYSNFN YFVKHISNSS 

       430        440        450        460        470        480 
KTLLHVLEGS HWLSAKISKK FSSKPIFLIG DSSSLLKGSL IVPLFNFTNV ICDNWNGLNI 

       490        500        510        520        530        540 
ISNDSSYLST KEFNLSSSHS QNSHLLNFPI NFVLNYDKAV LVDSSAFQIY QGHHGDTNAI 

       550        560        570        580        590        600 
NSNLIFPSTS FIEKNSFYSN SLAIVQKTKK ILFSPGNSRD DWKILNALID NFGFSYFKVR 

       610        620        630        640        650        660 
NSFDLVSFLS ESTPFILYKR SFSFKCFGFY EQLVYHFFNY FSVNNNYYIY DSITRNSKIM 

       670 
SLCFNKFKMK GYNFF

Q37373 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools