NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Thiel J., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.;
"Complete sequence of Prochlorococcus marinus str. MIT 9312.";
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000111; ABB50016.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_397452.1; -.

3D structure databases

ModBase

Q31AS9.

Enzyme and pathway databases

BioCyc

PMAR74546:PMT9312_0956-MON; -.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

Q31AS9.

Genome annotation databases

GeneID

3765759; -.

GenomeReviews

CP000111_GR; PMT9312_0956.

KEGG

pmi:PMT9312_0956; -.

Phylogenomic databases

HOGENOM

Q31AS9; -.

Genome annotation databases

CMR

Q31AS9; PMT9312_0956.

Other

ProtoNet

Q31AS9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 390`	`390`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_1000070548`

Sequence information

Length: 390 AA [This is the length of the unprocessed precursor]

Molecular weight: 45810 Da [This is the MW of the unprocessed precursor]

CRC64: 8013C443F31AF3B2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSQSIIESKN KKDVNNGKIP AKETILSPRF YTTDFEAMEN MDLSINEEEL EAICEEFRKD 

        70         80         90        100        110        120 
YNRHHFVRNS EFDGAAEKLD PETRELFVDF LEGSCTSEFS GFLLYKELSR RIKDKNPLLA 

       130        140        150        160        170        180 
ECFAHMARDE ARHAGFLNKS MSDFGLQLDL GFLTANKDYT YFPPRSIFYA TYLSEKIGYW 

       190        200        210        220        230        240 
RYIAIYRHLE KNPDSKIFPL FNYFENWCQD ENRHGDFFDA LMKAQPRTVK SLSQKINIGG 

       250        260        270        280        290        300 
TTFTHPLFDY FHRFRYFLNN LPITSKLWSR FFLLAVFATM YARDLGIKKD FYSSLGLDAR 

       310        320        330        340        350        360 
EYDQYVINKT NETSARVFPV VLDVYDKSFY GRLDKIVENN KVLSNIANSE ENKVSKTLKK 

       370        380        390 
LPTYLSNGYQ LLRLYLLKPL DSKDFQPSIR

Q31AS9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools