FUNCTION: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone (By similarity).
CATALYTIC ACTIVITY: Cholesterol + reduced adrenal ferredoxin + O₂ = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Lipid metabolism; C21-steroid hormone metabolism .
SUBCELLULAR LOCATION: Mitochondrion membrane (By similarity).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ228169; ABB76810.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q2XV99.

Ontologies

GO:0031966;	Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0008386;	Molecular function: cholesterol monooxygenase (side-chain-cleaving) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q2XV99.

Phylogenomic databases

HOVERGEN

Q2XV99; -.

Other

ProtoNet

Q2XV99.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol metabolism; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Mitochondrion; Monooxygenase; Oxidoreductase; Steroid metabolism; Steroidogenesis; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 39`	`39`	`Mitochondrion (By similarity).`
`CHAIN`	`40 521`	`482`	`Cytochrome P450 11A1, mitochondrial.`	`PRO_0000045764`
`METAL`	`462 462`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 521 AA [This is the length of the unprocessed precursor]

Molecular weight: 60242 Da [This is the MW of the unprocessed precursor]

CRC64: 91B747E6AC00BA20 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLAKGLPPRS VLVKGCQTFL SAPKERLGHL RVPTSEGAGI STRSPRPFNE IPSPGDNGWL 

        70         80         90        100        110        120 
NLYHFWRETG THKVHLHHVQ NFQKYDPIYR EKLGNVESVY VIDPEDVALL FKSEGPNPER 

       130        140        150        160        170        180 
FLIPPWVAYH QYYQRPIGVL LKKSAAWKKD RVALNQEVMA PETTKNFLPL LDAVSRDFVS 

       190        200        210        220        230        240 
VLHRRIKKAG SGNFSGDISD DLFRFAFESI TNVIFGERQG MLEEVVNPEG QRFIDAIYQM 

       250        260        270        280        290        300 
FHTSVHMLNL PPDLFRLFRT KTWKDHVAPR DVIFSKADMY TENFHWELRQ KGNVHHDYRG 

       310        320        330        340        350        360 
ILYRLLGDSK MSFEDIKANV TEMLAGGVDT TSMTLQWHLY EMARNLKVQD MLRAEVLAAR 

       370        380        390        400        410        420 
RQAQGDMATI LQLVPLLKAS IKETLRLHPI SVTLQRYLVN DLVLRGYMIP AKTLVQVAIY 

       430        440        450        460        470        480 
ALGREPTFFF DPENFDPTRW LSKDKNITYF RNLGFGWGVR QCLGRRIAEL EMTIFLINML 

       490        500        510        520 
ENFRVEIQHL SDVGTTFNLI LMPEKPISFT FWPFNQEATQ E

Q2XV99 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools