[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Gloeckner G., Schaap P., Winckler T.; "Evolutionary trends found in social amoebae."; Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
SUBUNIT: Complex I is composed of about 45 different subunits (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane (By similarity). Note=Matrix and cytoplasmic side of the mitochondrial inner membrane (By similarity).
SIMILARITY: Belongs to the complex I 75 kDa subunit family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ336395; ABC60398.4; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_492647.1; -.

3D structure databases

ModBase

Q2LCP5.

Family and domain databases

InterPro

IPR001041; Ferredoxin.
IPR006656; Mopterin_OxRdtase.
IPR000283; NADH_DHase_75KDa_su_CS.
IPR010228; NADH_quinone_OxRdtase_G.
Graphical view of domain structure.

Pfam

PF00111; Fer2; 1.
PF00384; Molybdopterin; 1.
Pfam graphical view of domain structure.

PROSITE

PS00197; 2FE2S_FER_1; FALSE_NEG.
PS51085; 2FE2S_FER_2; 1.
PS00641; COMPLEX1_75K_1; 1.
PS00642; COMPLEX1_75K_2; 1.
PS00643; COMPLEX1_75K_3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q2LCP5.

Genome annotation databases

GeneID

3912635; -.

Other

ProtoNet

Q2LCP5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 4Fe-4S; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transport; Ubiquinone.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 688`	`688`	`NADH-ubiquinone oxidoreductase 75 kDa subunit.`	`PRO_0000327378`
`DOMAIN`	`1 85`	`85`	`2Fe-2S ferredoxin-type.`
`METAL`	`38 38`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`49 49`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`52 52`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`66 66`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`101 101`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`105 105`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`108 108`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`114 114`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`153 153`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`156 156`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`159 159`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`
`METAL`	`204 204`		`Iron-sulfur 3 (4Fe-4S) (By similarity).`

Sequence information

Length: 688 AA [This is the length of the unprocessed precursor]

Molecular weight: 79605 Da [This is the MW of the unprocessed precursor]

CRC64: FDC7CCB227832A0C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLIRFKINEI ECEVDEEKED LTILQACTAN GIEIPRFCYH EKLTIAGNCR MCLVYVTNEE 

        70         80         90        100        110        120 
KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN HPLDCPICDQ GGECDLQEQT 

       130        140        150        160        170        180 
IAYGLDTGRF YIKKRAVEVK AFGRLIKGIM TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN 

       190        200        210        220        230        240 
MEIGTYKKNV IIESELSGNI IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTVLTPIN 

       250        260        270        280        290        300 
YQVKGGEIFR ILPRINDRLN EEWITDKVRF HYESYKIIEK MRKDTPSYKI QANKFIELSW 

       310        320        330        340        350        360 
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN GLLFKKFNYD 

       370        380        390        400        410        420 
FRENYINSND LYNVDQNDLV LLCGINLRVE SPLLNIKLRN INFGDDEIET RKKIGIIGNK 

       430        440        450        460        470        480 
FDWKQESEYL GSTVNSMLKV FEGRFPYCQQ IKKSKAPLIL VGSSLLSRIA ISLQEIRAAF 

       490        500        510        520        530        540 
EIASNIKPEN VLLITQGANF GMALEEGIFK ENFSKGGNVL YSIDSNEYKV SKTINYIIYQ 

       550        560        570        580        590        600 
GILNDTFENK IDLYLPSKHY FEDFESDREI YVNTFGQRSE IEKLRISKGN KIKENSMIGY 

       610        620        630        640        650        660 
IQLMYLNKKE MNRKEKEQKE IKITYRGIKQ KEKRQVKINK NLTINNIIDN YYMTDINSRL 

       670        680 
SKNLMITGQL RKEKKIMEAG SWKNKTCI

Q2LCP5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools