NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.01927-05; PubMed=16885469 [NCBI, ExPASy, EBI, Israel, Japan]
Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M.;
"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction.";
J. Bacteriol. 188:6002-6015(2006).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AM167904; CAJ50063.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_786965.1; -.

3D structure databases

ModBase

Q2KXN2.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

Q2KXN2.

Genome annotation databases

GeneID

6265985; -.

GenomeReviews

AM167904_GR; BAV2453.

KEGG

bav:BAV2453; -.

NMPDR

fig|521.1.peg.36; -.

Phylogenomic databases

HOGENOM

Q2KXN2; -.

Genome annotation databases

CMR

Q2KXN2; BAV2453.

Other

ProtoNet

Q2KXN2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 273`	`273`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000247703`

Sequence information

Length: 273 AA [This is the length of the unprocessed precursor]

Molecular weight: 30350 Da [This is the MW of the unprocessed precursor]

CRC64: 6B7132861004BE2D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPLSDAPLGH NVAYPSEYNA ALLFPIARAE NRAGLGLDGA LPFDGTDIWN AYELSWLDAK 

        70         80         90        100        110        120 
GKPRIAMASF RIPASSPNII ESKSFKLYLN SFNQTRLPNA QTLRDLLEKD LSAAAGAPVD 

       130        140        150        160        170        180 
MDFILPQRFD TLRIQELSGI NLDKLDVEVD RYEPAPELLG CTGTGIIEET LMSRLLKSNC 

       190        200        210        220        230        240 
PVTGQPDWAS VQIAYRGRPI DRAGLLKYII SFRQHAEFHE HCVERIFCDL MQACQPEQLT 

       250        260        270 
VYARYTRRGG LDINPWRSNA GASAPADLRG ARQ

Q2KXN2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools