[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Ascending colon; NIH - Mammalian Gene Collection (MGC) project; Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: 3-hydroxy-2-methylpropanoate + NAD⁺ = 2-methyl-3-oxopropanoate + NADH.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC105543; AAI05544.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001039571.1; -.

UniGene

Bt.23720

3D structure databases

SMR

Q2HJD7; 41-335.

ModBase

Q2HJD7.

Family and domain databases

InterPro

IPR002204; 3-OH-isobutyrate_DHase-rel_CS.
IPR015815; 3hydroxyacid_DHase/Rdtase.
IPR006183; 6-phosphogluconate_DHase.
IPR006115; 6PGDH_NAD-bd.
IPR013328; DHase_multihelical.
IPR011548; IsoBut3OH_DH.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.

PANTHER

PTHR22981; 3hydroxy_acid_DH; 1.

Pfam

PF03446; NAD_binding_2; 1.
Pfam graphical view of domain structure.

PRINTS

PR00076; 6PGDHDRGNASE.

TIGRFAMs

TIGR01692; HIBADH; 1.

PROSITE

PS00895; 3_HYDROXYISOBUT_DH; FALSE_NEG.

BLOCKS

Q2HJD7.

Genome annotation databases

Ensembl

ENSBTAG00000001036; Bos taurus. [Contig view]

GeneID

512002; -.

KEGG

bta:512002; -.

Phylogenomic databases

HOVERGEN

Q2HJD7; -.

Other

ProtoNet

Q2HJD7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; NAD; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 36`	`36`	`Mitochondrion (By similarity).`
`CHAIN`	`37 336`	`300`	`3-hydroxyisobutyrate dehydrogenase, mitochondrial.`	`PRO_0000290342`
`NP_BIND`	`40 69`	`30`	`NAD (By similarity).`
`NP_BIND`	`103 104`	`2`	`NAD (By similarity).`
`ACT_SITE`	`209 209`		`By similarity.`
`BINDING`	`108 108`		`NAD (By similarity).`
`BINDING`	`134 134`		`NAD; via amide nitrogen (By similarity).`
`BINDING`	`284 284`		`NAD (By similarity).`

Sequence information

Length: 336 AA [This is the length of the unprocessed precursor]

Molecular weight: 35410 Da [This is the MW of the unprocessed precursor]

CRC64: 11AB54F01BA8D5B8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAASLRLRGA ASGLRYWSRR QPPAVASLAA VCSRSMASKT PVGFIGVGNM GNPMAKNLMK 

        70         80         90        100        110        120 
HGYPLIIYDV FPDACKEFLD AGEQVVSSPA DVAEKADRII TMLPTSINAI EAYSGANGIL 

       130        140        150        160        170        180 
KKVKKGSLLI DSSTIDPMVS KELAKEVEKM GAVFMDAPVS GGVGAARSGN LTFMVGGVEE 

       190        200        210        220        230        240 
EFAAAQELLG CMGSNVVYCG AVGTGQAAKI CNNLLLAISM IGTAEAMNLG IRLGLDPKLL 

       250        260        270        280        290        300 
AKILNMSSGR CWSSDTYNPV PGVMDGVPSA NNYQGGFGTT LMAKDLGLAQ DSATSTKSPI 

       310        320        330 
LLGSQAHQIY RMMCAKGYSK KDFSSVFQFL REEETF

Q2HJD7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools