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UniProtKB/Swiss-Prot entry Q28943

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DPYD_PIG
Primary accession number Q28943
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on November 1, 1997 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 69)
Name and origin of the protein
Protein name Dihydropyrimidine dehydrogenase [NADP+] [Precursor]
Synonyms DHPDHase
DPD
EC 1.3.1.2
Dihydrouracil dehydrogenase
Dihydrothymine dehydrogenase
Gene name
Name: DPYD
From
Sus scrofa (Pig) [TaxID: 9823] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae; Sus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Liver;
PubMed=8083224 [NCBI, ExPASy, EBI, Israel, Japan]
Yokota H., Fernandez-Salguero P., Furuya H., Lin K., McBride O.W., Podschun B., Schnackerz K.D., Gonzalez F.J.;
"cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria.";
J. Biol. Chem. 269:23192-23196(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U09179; AAA57475.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B54718; B54718.
RefSeq NP_999209.1; -.
UniGene Ssc.153
3D structure databases
PDB
1GT8; X-ray; 3.30 A; A/B/C/D=1-1025.[ExPASy / RCSB / EBI]
1GTE; X-ray; 1.65 A; A/B/C/D=1-1025.[ExPASy / RCSB / EBI]
1GTH; X-ray; 2.25 A; A/B/C/D=1-1025.[ExPASy / RCSB / EBI]
1H7W; X-ray; 1.90 A; A/B/C/D=1-1025.[ExPASy / RCSB / EBI]
1H7X; X-ray; 2.01 A; A/B/C/D=1-1025.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1GT8; -.
1GTE; -.
1GTH; -.
1H7W; -.
1H7X; -.
ModBase Q28943.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from sequence or structural similarity from UniProtKB).
GO:0017113; Molecular function: dihydropyrimidine dehydrogenase (NADP+) activity (inferred from direct assay from UniProtKB).
GO:0050660; Molecular function: FAD binding (inferred from direct assay from UniProtKB).
GO:0010181; Molecular function: FMN binding (inferred from direct assay from UniProtKB).
GO:0050661; Molecular function: NADP binding (inferred from direct assay from UniProtKB).
GO:0042803; Molecular function: protein homodimerization activity (inferred from direct assay from UniProtKB).
GO:0006214; Biological process: thymidine catabolic process (inferred from sequence or structural similarity from UniProtKB).
GO:0006212; Biological process: uracil catabolic process (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR001450; 4Fe4S_Fe_S_bd.
IPR000759; Adrndx_reductase.
IPR013785; Aldolase_TIM.
IPR005720; DHO_DHase_1_core.
IPR001295; Dihydroorotate_DHase_core.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR012285; Fum_reductase_C.
IPR016040; NAD(P)-bd.
IPR001327; Pyr_OxRdtase_NAD_bd.
IPR000103; Pyridine_nuc-diS_OxRdtase_2.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.70; Aldolase_TIM; 1.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF01180; DHO_dh; 1.
PF00037; Fer4; 1.
PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
Pfam graphical view of domain structure.
PRINTS PR00353; 4FE4SFRDOXIN.
PR00419; ADXRDTASE.
PR00368; FADPNR.
PR00469; PNDRDTASEII.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01037; pyrD_sub1_fam; 1.
PROSITE PS00198; 4FE4S_FER_1; 1.
PS51379; 4FE4S_FER_2; 3.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q28943.
Genome annotation databases
GeneID 397109; -.
KEGG ssc:397109; -.
Phylogenomic databases
HOVERGEN Q28943; -.
Other
DrugBank DB00544; Fluorouracil.
ProtoNet Q28943.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; 4Fe-4S; Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; FMN; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
PROPEP   1      3  3      PRO_0000021116
CHAIN   4   1025  1022     Dihydropyrimidine dehydrogenase [NADP+]. PRO_0000021117
DOMAIN   69    100  32     4Fe-4S ferredoxin-type 1. 
DOMAIN   944    976  33     4Fe-4S ferredoxin-type 2. 
DOMAIN   978   1007  30     4Fe-4S ferredoxin-type 3. 
NP_BIND   335    351  17     NADP (Potential). 
NP_BIND   471    481  11     FAD (Potential). 
REGION   661    678  18     Uracil binding (Potential). 
METAL   953    953        Iron-sulfur 1 (4Fe-4S) (Potential). 
METAL   956    956        Iron-sulfur 1 (4Fe-4S) (Potential). 
METAL   959    959        Iron-sulfur 1 (4Fe-4S) (Potential). 
METAL   963    963        Iron-sulfur 1 (4Fe-4S) (Potential). 
METAL   986    986        Iron-sulfur 2 (4Fe-4S) (Potential). 
METAL   989    989        Iron-sulfur 2 (4Fe-4S) (Potential). 
METAL   992    992        Iron-sulfur 2 (4Fe-4S) (Potential). 
METAL   996    996        Iron-sulfur 2 (4Fe-4S) (Potential). 
MOD_RES   577    577        Phosphoserine (By similarity). 
HELIX   10     15  6      
TURN   16     18  3      
STRAND   24     26  3      
HELIX   31     41  11      
HELIX   69     78  10      
HELIX   86     89  4      
HELIX   97    105  9      
HELIX   109    119  11      
HELIX   123    129  7      
HELIX   132    134  3      
HELIX   136    139  4      
HELIX   141    144  4      
HELIX   152    166  15      
HELIX   179    181  3      
HELIX   184    187  4      
STRAND   190    193  4      
HELIX   197    208  12      
STRAND   214    222  9      
HELIX   226    229  4      
TURN   233    235  3      
HELIX   238    249  12      
TURN   250    252  3      
STRAND   254    258  5      
HELIX   268    273  6      
STRAND   278    281  4      
HELIX   292    294  3      
TURN   299    302  4      
STRAND   303    305  3      
HELIX   306    317  12      
STRAND   333    338  6      
HELIX   342    353  12      
STRAND   357    362  6      
HELIX   367    369  3      
HELIX   374    382  9      
STRAND   386    388  3      
STRAND   390    399  10      
STRAND   402    413  12      
STRAND   419    430  12      
STRAND   432    436  5      
HELIX   445    450  6      
TURN   451    453  3      
STRAND   460    462  3      
TURN   466    468  3      
STRAND   476    478  3      
HELIX   481    483  3      
HELIX   489    510  22      
HELIX   527    530  4      
STRAND   535    537  3      
STRAND   540    548  9      
HELIX   552    554  3      
HELIX   557    566  10      
STRAND   569    572  4      
HELIX   579    581  3      
STRAND   590    592  3      
STRAND   607    609  3      
HELIX   618    631  14      
STRAND   635    641  7      
HELIX   647    659  13      
STRAND   663    668  6      
HELIX   684    686  3      
HELIX   688    701  14      
STRAND   706    710  5      
HELIX   717    727  11      
STRAND   730    734  5      
STRAND   738    740  3      
STRAND   750    752  3      
TURN   755    758  4      
STRAND   763    767  5      
HELIX   768    770  3      
HELIX   771    784  14      
STRAND   790    795  6      
HELIX   799    807  9      
STRAND   811    816  6      
HELIX   817    820  4      
HELIX   826    840  15      
HELIX   844    846  3      
TURN   869    873  5      
HELIX   880    899  20      
HELIX   921    924  4      
TURN   925    928  4      
HELIX   929    931  3      
HELIX   935    937  3      
STRAND   940    942  3      
STRAND   944    948  5      
TURN   950    952  3      
HELIX   958    966  9      
STRAND   971    973  3      
TURN   975    977  3      
STRAND   980    982  3      
HELIX   991    995  5      
TURN   999   1001  3      
STRAND   1002   1006  5      
Sequence information
Length: 1025 AA [This is the length of the unprocessed precursor] Molecular weight: 111424 Da [This is the MW of the unprocessed precursor] CRC64: B8D553AD862845D0 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAPVLSKDVA DIESILALNP RTQSHAALHS TLAKKLDKKH WKRNPDKNCF HCEKLENNFG 

        70         80         90        100        110        120 
DIKHTTLGER GALREAMRCL KCADAPCQKS CPTHLDIKSF ITSISNKNYY GAAKMIFSDN 

       130        140        150        160        170        180 
PLGLTCGMVC PTSDLCVGGC NLYATEEGSI NIGGLQQFAS EVFKAMNIPQ IRNPCLPSQE 

       190        200        210        220        230        240 
KMPEAYSAKI ALLGAGPASI SCASFLARLG YSDITIFEKQ EYVGGLSTSE IPQFRLPYDV 

       250        260        270        280        290        300 
VNFEIELMKD LGVKIICGKS LSENEITLNT LKEEGYKAAF IGIGLPEPKT DDIFQGLTQD 

       310        320        330        340        350        360 
QGFYTSKDFL PLVAKSSKAG MCACHSPLPS IRGAVIVLGA GDTAFDCATS ALRCGARRVF 

       370        380        390        400        410        420 
LVFRKGFVNI RAVPEEVELA KEEKCEFLPF LSPRKVIVKG GRIVAVQFVR TEQDETGKWN 

       430        440        450        460        470        480 
EDEDQIVHLK ADVVISAFGS VLRDPKVKEA LSPIKFNRWD LPEVDPETMQ TSEPWVFAGG 

       490        500        510        520        530        540 
DIVGMANTTV ESVNDGKQAS WYIHKYIQAQ YGASVSAKPE LPLFYTPVDL VDISVEMAGL 

       550        560        570        580        590        600 
KFINPFGLAS AAPTTSSSMI RRAFEAGWGF ALTKTFSLDK DIVTNVSPRI VRGTTSGPMY 

       610        620        630        640        650        660 
GPGQSSFLNI ELISEKTAAY WCQSVTELKA DFPDNIVIAS IMCSYNKNDW MELSRKAEAS 

       670        680        690        700        710        720 
GADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRQ AVQIPFFAKL TPNVTDIVSI 

       730        740        750        760        770        780 
ARAAKEGGAD GVTATNTVSG LMGLKADGTP WPAVGAGKRT TYGGVSGTAI RPIALRAVTT 

       790        800        810        820        830        840 
IARALPGFPI LATGGIDSAE SGLQFLHSGA SVLQVCSAVQ NQDFTVIQDY CTGLKALLYL 

       850        860        870        880        890        900 
KSIEELQGWD GQSPGTESHQ KGKPVPRIAE LMGKKLPNFG PYLEQRKKII AEEKMRLKEQ 

       910        920        930        940        950        960 
NAAFPPLERK PFIPKKPIPA IKDVIGKALQ YLGTFGELSN IEQVVAVIDE EMCINCGKCY 

       970        980        990       1000       1010       1020 
MTCNDSGYQA IQFDPETHLP TVTDTCTGCT LCLSVCPIID CIRMVSRTTP YEPKRGLPLA 


VNPVC
Q28943 in FASTA format

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View entry in raw text format (no links)
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