[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Parietal cortex; International consortium for macaque cDNA sequencing and analysis; "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."; Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 3-385. TISSUE=Testis; PubMed=7575427 [NCBI, ExPASy, EBI, Israel, Japan] Nichols B.J., Perry A.C.F., Hall L., Denton R.M.; "Molecular cloning and deduced amino acid sequences of the alpha- and beta-subunits of mammalian NAD(+)-isocitrate dehydrogenase."; Biochem. J. 310:917-922(1995).

Comments

CATALYTIC ACTIVITY: Isocitrate + NAD⁺ = 2-oxoglutarate + CO₂ + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB169839; BAE01920.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X82632; CAA57954.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S58663; S58663.

3D structure databases

HSSP

P39126; 1HQS. [HSSP ENTRY / PDB]

ModBase

Q28479.

Family and domain databases

InterPro

IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00175; mito_nad_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

Q28479.

Phylogenomic databases

HOVERGEN

Q28479; -.

Other

ProtoNet

Q28479.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 34`	`34`	`Mitochondrion (By similarity).`
`CHAIN`	`35 385`	`351`	`Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial.`	`PRO_0000014445`
`NP_BIND`	`50 78`	`29`	`NAD (Potential).`
`METAL`	`251 251`		`Magnesium or manganese (By similarity).`
`BINDING`	`133 133`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`251 251`		`Substrate (By similarity).`
`SITE`	`171 171`	`1`	`Critical for catalysis (By similarity).`
`SITE`	`218 218`	`1`	`Critical for catalysis (By similarity).`
`CONFLICT`	`361 361`		`R -> S (in Ref. 2; CAA57954).`
`CONFLICT`	`367 385`		`STTTDFIKSVIGHLHPHGS -> ATCHDFTEAVIAALPHP (in Ref. 2; CAA57954).`

Sequence information

Length: 385 AA [This is the length of the unprocessed precursor]

Molecular weight: 42255 Da [This is the MW of the unprocessed precursor]

CRC64: 1730F0E614595EB3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAALSGVRWL TRALVSAGNP GAWRGLSTSA AAHAASRSQA EDVRVEGSFP VTMLPGDGVG 

        70         80         90        100        110        120 
PELMHAVKEV FKAAAVPVEF QEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIHTPME 

       130        140        150        160        170        180 
YKGELASYDM RLRRKLDLFA NVVHVKSLPG YMTRHNNLDL VIIREQTEGE YSSLEHESAR 

       190        200        210        220        230        240 
GVIECLKIVT RAKSQRIAKF AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY 

       250        260        270        280        290        300 
PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 

       310        320        330        340        350        360 
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSNMIADAVK KVIKVGKVRT 

       370        380 
RDMGGYSTTT DFIKSVIGHL HPHGS

Q28479 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools