NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Peripheral blood monocyte;
PubMed=8603979 [NCBI, ExPASy, EBI, Israel, Japan]
Adler H., Adler B., Peveri P., Werner E.R., Wachter H., Peterhans E., Jungi T.W.;
"Differential regulation of inducible nitric oxide synthase production in bovine and caprine macrophages.";
J. Infect. Dis. 173:971-978(1996).

Comments

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O₂ = citrulline + nitric oxide + n NADP⁺.
COFACTOR: Heme group (By similarity).
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
COFACTOR: Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
ENZYME REGULATION: Regulated by calcium/calmodulin (By similarity).
SUBUNIT: Homodimer. Binds SLC9A3R1 (By similarity).
INDUCTION: By lipopolysaccharide (LPS).
SIMILARITY: Belongs to the NOS family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U29085; AAB02338.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P35228; 4NOS. [HSSP ENTRY / PDB]

SMR

Q28314; 1-110.

ModBase

Q28314.

Family and domain databases

InterPro

IPR004030; NO_synthase_oxygenase_reg.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.340.10; NO_synthase_oxygenase_reg; 1.

Pfam

PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.

PROSITE

PS60001; NOS; 1.

BLOCKS

Q28314.

Phylogenomic databases

HOVERGEN

Q28314; -.

Other

ProtoNet

Q28314.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Calmodulin-binding; FAD; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >110`	`>110`	`Nitric oxide synthase, inducible.`	`PRO_0000170928`
`METAL`	`35 35`		`Iron (heme axial ligand) (By similarity).`
`NON_TER`	`1 1`
`NON_TER`	`110 110`

Sequence information

Length: 110 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 12654 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: D72188FB257EC518 [This is a checksum on the sequence]

        10         20         30         40         50         60 
VEAVTKEIET TGTYQLTGDE LIFATKQAWR NAPRCIGRIQ WSNLQVFDAR SCSTAQEMFE 

        70         80         90        100        110 
HICRHVRYAT NNGNIRSAIT VFPQRSDGKH DFRVWNAQLI RYAGYQMPDG

Q28314 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools