[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Brain, and Liver; PubMed=1378441 [NCBI, ExPASy, EBI, Israel, Japan] Jia S., Vandusen W.J., Diehl R.E., Kohl N.E., Dixon R.A.F., Elliston K.O., Stern A.M., Friedman P.A.; "cDNA cloning and expression of bovine aspartyl (asparaginyl) beta-hydroxylase."; J. Biol. Chem. 267:14322-14327(1992).
[2]	PROTEIN SEQUENCE OF 289-385 AND 615-641. TISSUE=Liver; PubMed=1856229 [NCBI, ExPASy, EBI, Israel, Japan] Wang Q., Vandusen W.J., Petroski C.J., Garsky V.M., Stern A.M., Friedman P.A.; "Bovine liver aspartyl beta-hydroxylase. Purification and characterization."; J. Biol. Chem. 266:14004-14010(1991).

Comments

FUNCTION: Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.
CATALYTIC ACTIVITY: Peptide L-aspartate + 2-oxoglutarate + O₂ = peptide 3-hydroxy-L-aspartate + succinate + CO₂.
COFACTOR: Iron.
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type II membrane protein.
PTM: Might be processed to the 56 kDa (AA 289-754) or 52 kDa (AA 311-754) forms in the lumen of the endoplasmic reticulum.
SIMILARITY: Belongs to the aspartyl/asparaginyl beta-hydroxylase family.
SIMILARITY: Contains 5 TPR repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M91213; AAA03563.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

A42969; BABOH.

NP_777182.1; -.

3D structure databases

ModBase

Q28056.

Family and domain databases

InterPro

IPR007943; Asp-B-hydro_N.
IPR007803; Asp_Arg_Hydrox.
IPR011990; TPR-like_helical.
IPR013026; TPR_region.
Graphical view of domain structure.

Gene3D

G3DSA:1.25.40.10; TPR-like_helical; 1.

Pfam

PF05279; Asp-B-Hydro_N; 1.
PF05118; Asp_Arg_Hydrox; 1.
Pfam graphical view of domain structure.

PROSITE

PS50005; TPR; 2.
PS50293; TPR_REGION; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q28056.

Genome annotation databases

Ensembl

ENSBTAG00000026283; Bos taurus. [Contig view]

GeneID

286771; -.

KEGG

bta:286771; -.

Phylogenomic databases

HOVERGEN

Q28056; -.

Other

ProtoNet

Q28056.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Iron; Membrane; Oxidoreductase; Phosphoprotein; Repeat; Signal-anchor; TPR repeat; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 754`	`754`	`Aspartyl/asparaginyl beta-hydroxylase.`	`PRO_0000064705`
`TOPO_DOM`	`1 56`	`56`	`Cytoplasmic (Potential).`
`TRANSMEM`	`57 77`	`21`	`Signal-anchor for type II membrane protein (Potential).`
`TOPO_DOM`	`78 754`	`677`	`Lumenal (Potential).`
`REPEAT`	`337 370`	`34`	`TPR 1.`
`REPEAT`	`378 411`	`34`	`TPR 2.`
`REPEAT`	`450 483`	`34`	`TPR 3.`
`REPEAT`	`485 517`	`33`	`TPR 4.`
`REPEAT`	`521 553`	`33`	`TPR 5.`
`COMPBIAS`	`9 12`	`4`	`Poly-Gly.`
`COMPBIAS`	`14 21`	`8`	`Poly-Ser.`
`COMPBIAS`	`311 352`	`42`	`Lys-rich.`
`MOD_RES`	`32 32`		`Phosphoserine (By similarity).`
`CARBOHYD`	`96 96`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`466 466`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`702 702`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 754 AA [This is the length of the unprocessed precursor]

Molecular weight: 84999 Da [This is the MW of the unprocessed precursor]

CRC64: 369593A1F0B558C8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAPRKNAKGG GGNSSSSSSG SPTGCTSGGS SSPGARRETK QGGLKNGRKG GLSGSSFFTW 

        70         80         90        100        110        120 
FMVIALLGVW TSVAVVWFDL VDYEEVLAKA KDFRYNLSEV LQGKLGIYDA DGDGDFDVDD 

       130        140        150        160        170        180 
AKVLLGLKEK PAPKPTVPPE EADMYPWLED QVLESPGRQN IEDEVYEQVQ SLDETVYSEP 

       190        200        210        220        230        240 
GENLPQEPEG PAEELQPDDH VFVGSDADDR YEPMGTGAVH EETEDSYHIE ETASPAYSQD 

       250        260        270        280        290        300 
MEDMMYEQEN PDSSEPVVVD DAERTYQETD DVTYRDYDEQ DHAVDNSNTI LEEPHMPPAE 

       310        320        330        340        350        360 
EQQEVPPETN KKADEPGKKG KVKKKKPKLL NKFDKTIKAE LDAAEKLRKR GKIEEAVNAF 

       370        380        390        400        410        420 
EELVRKYPQS PGARYGKAQC EDDLAEKRRS NEILRRAIET YQEAASLPDA PTDLVKLSLK 

       430        440        450        460        470        480 
RRSDRQQFLG HMRGSLLTLQ KLVQLFPDDT ALKNDLGVGY LLIGDNDSAK KVYEEVLSVT 

       490        500        510        520        530        540 
PNDGFAKVHY GFILKAQNKI AESIPYLKEG IESGDPGTDD GRFYFHLGDA MQRVGNKEAY 

       550        560        570        580        590        600 
RWYELGHQRG HFASVWQRSL YNVQGLKAQP WWTPKETGYT ELVKSLERNW KLIRDEGLAA 

       610        620        630        640        650        660 
MDRTHGLFLP EDENLREKGD WSQFTLWQQG RKNENACKGA PKTCSLLDKF PETTGCRRGQ 

       670        680        690        700        710        720 
IKYSIMHPGT HVWPHTGPTN CRLRMHLGLV IPKEGCKIRC ANETRTWEEG KVLIFDDSFE 

       730        740        750 
HEVWQDAASF RLIFIVDVWH PELTPHQRRS LPAI

Q28056 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools