[1]	NUCLEOTIDE SEQUENCE [MRNA]. Widdison S., Ashley G.R., Howard C.J., Coffey T.J.; "Cloning and characterization of bovine inducible nitric oxide synthase."; Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 166-275. TISSUE=Bone marrow macrophage; PubMed=7536776 [NCBI, ExPASy, EBI, Israel, Japan] Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W.; "Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages."; J. Immunol. 154:4710-4718(1995).
[3]	NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122. TISSUE=Pulmonary artery; DOI=10.1006/geno.1995.1086; PubMed=7558036 [NCBI, ExPASy, EBI, Israel, Japan] Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B.; "Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17."; Genomics 27:526-530(1995).

Comments

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O₂ = citrulline + nitric oxide + n NADP⁺.
COFACTOR: Heme group (By similarity).
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
COFACTOR: Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
ENZYME REGULATION: Regulated by calcium/calmodulin (By similarity).
SUBUNIT: Homodimer. Binds SLC9A3R1 (By similarity).
SIMILARITY: Belongs to the NOS family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ676956; ABG74910.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U14640; AAC48470.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U18331; AAC48479.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

I46074; I46074.

NP_001070267.1; -.

3D structure databases

HSSP

P29477; 1DD7. [HSSP ENTRY / PDB]

SMR

Q27995; 83-502.

ModBase

Q27995.

Family and domain databases

InterPro

IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR004030; NO_synthase_oxygenase_reg.
IPR012144; NOS.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Pfam

PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000333; NOS; 1.

PRINTS

PR00369; FLAVODOXIN.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PS60001; NOS; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q27995.

Genome annotation databases

Ensembl

ENSBTAG00000006894; Bos taurus. [Contig view]

GeneID

282876; -.

KEGG

bta:282876; -.

Phylogenomic databases

HOVERGEN

Q27995; -.

Other

ProtoNet

Q27995.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Calmodulin-binding; FAD; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1156`	`1156`	`Nitric oxide synthase, inducible.`	`PRO_0000170926`
`DOMAIN`	`539 677`	`139`	`Flavodoxin-like.`
`DOMAIN`	`730 970`	`241`	`FAD-binding FR-type.`
`NP_BIND`	`623 654`	`32`	`FMN (By similarity).`
`NP_BIND`	`767 778`	`12`	`FAD (By similarity).`
`NP_BIND`	`903 913`	`11`	`FAD (By similarity).`
`NP_BIND`	`978 996`	`19`	`NADP (By similarity).`
`NP_BIND`	`1076 1091`	`16`	`NADP (By similarity).`
`REGION`	`509 529`	`21`	`Calmodulin-binding (Potential).`
`METAL`	`200 200`		`Iron (heme axial ligand) (By similarity).`
`CONFLICT`	`236 236`		`N -> S (in Ref. 2; AAC48470).`
`CONFLICT`	`1068 1069`		`VL -> AF (in Ref. 3; AAC48479).`

Sequence information

Length: 1156 AA [This is the length of the unprocessed precursor]

Molecular weight: 131208 Da [This is the MW of the unprocessed precursor]

CRC64: 52A52C984462F692 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG KHGNESPQPL 

        70         80         90        100        110        120 
TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL HQKAKGDLSC KSKSCLASIM 

       130        140        150        160        170        180 
NPKSLTIGPR DKPTPPDELL PQAIEFVNQY YGSFKEAKIE EHLARVEAVT KEIETTGTYQ 

       190        200        210        220        230        240 
LTGDELIFAT KQAWRNAPRC IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI 

       250        260        270        280        290        300 
RSAITVFPQR SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG 

       310        320        330        340        350        360 
RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP AVANMLLEVG 

       370        380        390        400        410        420 
GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG LETHKVASLW KDRAVVEINV 

       430        440        450        460        470        480 
AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE YRSRGGCPAD WIWLVPPISG SITPVFHQEM 

       490        500        510        520        530        540 
LNYVLSPFYY YQVEPWKTHV WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT 

       550        560        570        580        590        600 
ILFATETGRS ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG 

       610        620        630        640        650        660 
NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA SQLAPTGEGD 

       670        680        690        700        710        720 
ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS NVTWDPQHYR LVQDSEPLDL 

       730        740        750        760        770        780 
NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ 

       790        800        810        820        830        840 
PALVQGILER VVDGPAPHQP VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ 

       850        860        870        880        890        900 
LLLRKLAQLA TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL 

       910        920        930        940        950        960 
PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS LKPQDPVPCF 

       970        980        990       1000       1010       1020 
VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA EHKGLQGGRM TLVFGCRRPE 

      1030       1040       1050       1060       1070       1080 
EDHLYWEEML EMARKGVLHE VHTAYSRLPD QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY 

      1090       1100       1110       1120       1130       1140 
VCGDVRMARD VARTLKQLMA TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG 

      1150 
AAGLPSNPRA PGAHRS

Q27995 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools