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UniProtKB/Swiss-Prot entry Q27895

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NTP2_TOXGO
Primary accession number Q27895
Secondary accession numbers Q27798 Q27801
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on November 1, 1997 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 52)
Name and origin of the protein
Protein name Nucleoside-triphosphatase 2 [Precursor]
Synonyms EC 3.6.1.15
NTPase-II
Nucleoside-triphosphatase II
Nucleoside triphosphate hydrolase 2
Gene name
Name: NTP1
From
Toxoplasma gondii [TaxID: 5811] 
Taxonomy Eukaryota; Alveolata; Apicomplexa; Coccidia; Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
STRAIN=Beverley, and RH;
DOI=10.1074/jbc.270.19.11391; PubMed=7744775 [NCBI, ExPASy, EBI, Israel, Japan]
Asai T., Miura S., Sibley L.D., Okabayashi H., Takeuchi T.;
"Biochemical and molecular characterization of nucleoside triphosphate hydrolase isozymes from the parasitic protozoan Toxoplasma gondii.";
J. Biol. Chem. 270:11391-11397(1995).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=RH;
PubMed=7961894 [NCBI, ExPASy, EBI, Israel, Japan]
Bermudes D., Peck K.R., Afifi M.A., Beckers C.J.M., Joiner K.A.;
"Tandemly repeated genes encode nucleoside triphosphate hydrolase isoforms secreted into the parasitophorous vacuole of Toxoplasma gondii.";
J. Biol. Chem. 269:29252-29260(1994).
[3]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 334-523.
STRAIN=RH;
DOI=10.1016/0378-1119(89)90484-8; PubMed=2482826 [NCBI, ExPASy, EBI, Israel, Japan]
Johnson A.M., Illana S., McDonald P.J., Asai T.;
"Cloning, expression and nucleotide sequence of the gene fragment encoding an antigenic portion of the nucleoside triphosphate hydrolase of Toxoplasma gondii.";
Gene 85:215-220(1989).
Comments
  • FUNCTION: May perform an important processing step in the conversion of high energy nucleotides prior to uptake by the parasite. NTPAse-II has a specific activity 4.5-fold lower than NTPAse-I in hydrolysis of ATP. The primary difference between these isozymes lies in their ability to hydrolyze nucleoside triphosphate versus diphosphate substrates. While NTPAse-II hydrolyzes ATP to ADP and ADP to AMP at almost the same rate, NTPAse-I hydrolyzes ADP to AMP at a much slower rate (0.7% of the rate for ATP).
  • CATALYTIC ACTIVITY: NTP + H2O = NDP + phosphate.
  • SUBUNIT: Homotetramer.
  • SUBCELLULAR LOCATION: Secreted. Parasitophorous vacuole. Note=Found in host cell parasitophorous vacuole.
  • SIMILARITY: Belongs to the GDA1/CD39 NTPase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L39077; AAC41569.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L39079; AAC41570.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U96965; AAC80187.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M33472; AAA30143.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A55421; A55421.
3D structure databases
ModBase Q27895.
Ontologies
GO
GO:0020003; Cellular component: parasitophorous vacuole (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005773; Cellular component: vacuole (inferred from electronic annotation from UniProtKB-KW).
GO:0017111; Molecular function: nucleoside-triphosphatase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR000407; GDA1_CD39_NTPase.
IPR017227; NTPase.
Graphical view of domain structure.
PANTHER PTHR11782; GDA1_CD39_NTPase; 1.
Pfam PF01150; GDA1_CD39; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037506; NTPase; 1.
PROSITE PS01238; GDA1_CD39_NTPASE; 1.
BLOCKS Q27895.
ProtoNet Q27895.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Glycoprotein; Hydrolase; Secreted; Signal; Vacuole.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    25  25      
CHAIN   26   628  603     Nucleoside-triphosphatase 2. PRO_0000019912
CARBOHYD   432   432        N-linked (GlcNAc...) (Potential). 
VARIANT   6     6  1     Y -> H (in strain: Beverley). 
VARIANT   91    91  1     K -> R (in strain: Beverley). 
VARIANT   101   101  1     Q -> R (in strain: Beverley). 
CONFLICT   334   334        G -> R (in Ref. 3; AAA30143). 
CONFLICT   370   370        V -> L (in Ref. 3). 
CONFLICT   372   372        V -> L (in Ref. 3). 
CONFLICT   399   399        H -> N (in Ref. 3; AAA30143). 
CONFLICT   437   437        E -> K (in Ref. 3; AAA30143). 
CONFLICT   488   489        FI -> IV (in Ref. 3). 
CONFLICT   492   494        REM -> GGS (in Ref. 3). 
CONFLICT   497   497        S -> A (in Ref. 3). 
CONFLICT   499   499        D -> N (in Ref. 3). 
CONFLICT   523   523        R -> G (in Ref. 3; AAA30143). 
Sequence information
Length: 628 AA [This is the length of the unprocessed precursor] Molecular weight: 69585 Da [This is the MW of the unprocessed precursor] CRC64: DE943DFDAC641159 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MWLPVYVPLL LVFGVSLSLP HGSLGTDSSS LRGVDADTEK RINVGKTHLQ TLRNLETRCH 

        70         80         90        100        110        120 
DSLQALVVID AGSSSTRTNV FLAKTRSCPN KGRSIDPDSI QLIREGKRFT GLRVVLEEWL 

       130        140        150        160        170        180 
DTYAGKDWES RPVDARLLFQ YVPQMHEGAK KLMQLLEEDT VAILDSQLNE EQKVQVKALG 

       190        200        210        220        230        240 
IPVMLCSTAG VRDFHEWYRD ALFVLLRHLI NNPSPAHGYK FFTNPFWTRP ITGAEEGLFA 

       250        260        270        280        290        300 
FITLNHLSRR LGEDPARCMI DEYGVKHCRN DLAGVVEVGG ASAQIVFPLQ EGTVLPSSVR 

       310        320        330        340        350        360 
AVNLQRERLL PERYPSADVV SVSFMQLGMA SSAGLFLKEL CSNDEFLQGG ICSNPCLFKG 

       370        380        390        400        410        420 
FQQSCSAGEV EVRPDGSASV NEDVRKNRLK PLATYCSVHN PEISFKVTNE MQCRENSIDP 

       430        440        450        460        470        480 
TKPLAERMKI ENCSIIEGTG NFDKCVSQVE SILVAPKLPL PANIEAASSG FESVDQVFRF 

       490        500        510        520        530        540 
ASSTAPMFIT GREMLASIDT LKDHRLLRSD FSGDVEELAE AAREFCSSEV IIRTDGPVIQ 

       550        560        570        580        590        600 
LPNARGEQKL NSLNFDLCKT MALTVSLLRH MAAGENQPSF IKWEKSIAGP DGKPLADLGW 

       610        620 
QVGVILHHVL FTEEWGRTAY EAGYSHNL
Q27895 in FASTA format

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