NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=EATRO 164;
DOI=10.1016/0166-6851(93)90091-B; PubMed=8459836 [NCBI, ExPASy, EBI, Israel, Japan]
Peterson G.C., Souza A.E., Parsons M.;
"Characterization of a Trypanosoma brucei nuclear gene encoding a protein homologous to a subunit of bovine NADH:ubiquinone oxidoreductase (complex I).";
Mol. Biochem. Parasitol. 58:63-70(1993).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
SUBUNIT: Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the complex I 20 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L00585; AAA30217.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q26783.

Family and domain databases

InterPro

IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.

PANTHER

PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.

Pfam

PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01957; nuoB_fam; 1.

PROSITE

PS01150; COMPLEX1_20K; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 56`	`56`	`Mitochondrion (Potential).`
`CHAIN`	`57 202`	`146`	`NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial.`	`PRO_0000020031`
`METAL`	`77 77`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`78 78`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`142 142`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`172 172`		`Iron-sulfur (4Fe-4S) (Potential).`

Sequence information

Length: 202 AA [This is the length of the unprocessed precursor]

Molecular weight: 23077 Da [This is the MW of the unprocessed precursor]

CRC64: 58CC3911E359E50D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLRRTSFNFT GRAMISRGSP EWSHRLDLKK GKKTTMMHKL GTSKPNNALQ YAQMTLHDLT 

        70         80         90        100        110        120 
EWCLAYSPWP LTFGLACCAV EMMHAYASRY DLDRFGIVPR PTPRQAEIII VSGTVTNKMA 

       130        140        150        160        170        180 
PILRNIYVQM VNPKWVISMG SCANGGGYYH FSYAVLRGCE RAIPVDFWIP GCPPSAESLV 

       190        200 
FCLHTLQKKI RWHEIQKYSV RD

Q26783 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools