NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Salivary gland;
PubMed=9022713 [NCBI, ExPASy, EBI, Israel, Japan]
Yuda M., Hirai M., Miura K., Matsumura H., Ando K., Chinzei Y.;
"cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus.";
Eur. J. Biochem. 242:807-812(1996).

Comments

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. The production of NO in the salivary gland is used as a vasodilator for blood sucking.
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O₂ = citrulline + nitric oxide + n NADP⁺.
COFACTOR: Heme group (By similarity).
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
ENZYME REGULATION: Stimulated by calcium/calmodulin.
SIMILARITY: Belongs to the NOS family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U59389; AAB03810.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P29477; 1DD7. [HSSP ENTRY / PDB]

ModBase

Q26240.

Family and domain databases

InterPro

IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR004030; NO_synthase_oxygenase_reg.
IPR012144; NOS.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.340.10; NO_synthase_oxygenase_reg; 1.

Pfam

PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000333; NOS; 1.

PRINTS

PR00369; FLAVODOXIN.
PR00371; FPNCR.

PROSITE

PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PS60001; NOS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calmodulin-binding; FAD; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1174`	`1174`	`Nitric oxide synthase, salivary gland.`	`PRO_0000170952`
`DOMAIN`	`505 693`	`189`	`Flavodoxin-like.`
`DOMAIN`	`745 990`	`246`	`FAD-binding FR-type.`
`NP_BIND`	`639 670`	`32`	`FMN (By similarity).`
`NP_BIND`	`780 791`	`12`	`FAD (By similarity).`
`NP_BIND`	`923 933`	`11`	`FAD (By similarity).`
`NP_BIND`	`998 1016`	`19`	`NADP (By similarity).`
`NP_BIND`	`1095 1110`	`16`	`NADP (By similarity).`
`REGION`	`475 495`	`21`	`Calmodulin-binding (Potential).`
`COMPBIAS`	`35 45`	`11`	`Poly-Gln.`
`METAL`	`162 162`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 1174 AA [This is the length of the unprocessed precursor]

Molecular weight: 132393 Da [This is the MW of the unprocessed precursor]

CRC64: C32F664EE51409CF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVHAECVWWL GIRILFVPPV SLEMHSVNVN NMSIQQQQQH QPQQQLLKPI RLANVSTQAQ 

        70         80         90        100        110        120 
SLDTLHYKCQ QEGPCMEQAC LASVIYAGVN LKPRVRPKEE LLAHAKDFLD QYFASIRRLQ 

       130        140        150        160        170        180 
SPAHEARWAQ VEKEVAATGT YELTETELVY GAKLAWRNAP RCIGRIQWAK LQVFDCRQVT 

       190        200        210        220        230        240 
TTSGMFEALC NHIKYSTNKG NIRSAITIFP HRTDGKHDFR IWNKQLISYA GHKSKDGTVI 

       250        260        270        280        290        300 
GDPACVEFTE ICIKLGWKGK GTMFDVLPLV LSANGEDPDY FDLPPELVFE VPLSHPKYKW 

       310        320        330        340        350        360 
FSELGLKWFA LPAVSGMMFD CGGLQFTAAP FNGWYMNSEI GSRNLGDTNR YNMLEKIAQK 

       370        380        390        400        410        420 
MELDTRTPVT LWKDLAMVEA NVAVLHSFQL HNVTIVDHHS AAESFMKHLE NEQRLRGGCP 

       430        440        450        460        470        480 
ADWVWIVPPI SGSATPVFFQ EMANYFLYPG YIYQEDAWKC HEWKEIDVKH GLKKEKRKFH 

       490        500        510        520        530        540 
FKQIARAVKF TSKLFGSALS KRIKATILFA TETGKSEMYA RKLGDIFSHA FHSQVLSMED 

       550        560        570        580        590        600 
YDMSKIEHEA LLLVVASTFG NGDPPENGQG FAQSLYTIKM DENGLPNGHT NNTLASSASF 

       610        620        630        640        650        660 
IKANSQTDRQ ASLERCDSFR GSTGDADVFG PLSNVRFAVF ALGSSAYPNF CAFGSYVDNL 

       670        680        690        700        710        720 
LGELGGERLV KLTTGDEMCG QAQACNKWAP EVFSVACDTF CLDSDETFLE ATQMLHSEAV 

       730        740        750        760        770        780 
TASTVRFVES ATQDLCKALS HLHNKKVWKC PLLGKRNLHG KGSTRATLLL EIERNENISY 

       790        800        810        820        830        840 
QPGDHVGVLA CNRKELVEGI ISHLESAIDP DKSVQLQILK ENTTPDGIVR NWIPHDRLPT 

       850        860        870        880        890        900 
CSLRTMLTRF LDITTPPSPN LLQFFASCAT NSEDQEKLTE LATDSAAYED WRYWKYPNLL 

       910        920        930        940        950        960 
EVLEEFPSVR VLPALLIAQL TPLQPRFYSI SSAPSLYANQ IHLTVAVVQY CTQDGKGPIH 

       970        980        990       1000       1010       1020 
YGVASNYLYD VTIGDSIYLF TRSAPNFHLP KSDTAPIIMV GPGTGIAPFR GFWQHRLAQR 

      1030       1040       1050       1060       1070       1080 
SLNGPGKFGK MSLFFGCRLR NLDLYQEEKE SMLKEGILSK VFLALSREPS IPKTYVQDLL 

      1090       1100       1110       1120       1130       1140 
RVECKSVYIQ IVQEGGHFYV CGDCTMAEHV FRTLRQIIQD QGNMTDHQVD NFMLAMRDEN 

      1150       1160       1170 
RYHEDIFGIT LRTAEVHNRS RESARIRMAS QSQP

Q26240 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools