[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 377-395 AND 860-870. STRAIN=HM-1:IMSS; DOI=10.1016/0166-6851(93)00020-A; PubMed=7935603 [NCBI, ExPASy, EBI, Israel, Japan] Yang W., Li E., Kairong T., Stanley S.L. Jr.; "Entamoeba histolytica has an alcohol dehydrogenase homologous to the multifunctional adhE gene product of Escherichia coli."; Mol. Biochem. Parasitol. 64:253-260(1994).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=HM-1:IMSS; PubMed=7980441 [NCBI, ExPASy, EBI, Israel, Japan] Bruchhaus I., Tannich E.; "Purification and molecular characterization of the NAD(+)-dependent acetaldehyde/alcohol dehydrogenase from Entamoeba histolytica."; Biochem. J. 303:743-748(1994).

Comments

FUNCTION: This enzyme has two NAD(+)-dependent activities: ADH and ACDH. May be a critical enzyme in the fermentative pathway.
CATALYTIC ACTIVITY: An alcohol + NAD⁺ = an aldehyde or ketone + NADH.
CATALYTIC ACTIVITY: Acetaldehyde + CoA + NAD⁺ = acetyl-CoA + NADH.
COFACTOR: Zinc or iron.
SUBUNIT: Seems to form a rod shaped homopolymer composed of at least 20 identical subunits.
SIMILARITY: In the N-terminal section; belongs to the aldehyde dehydrogenase family.
SIMILARITY: In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U04863; AAA81906.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X77132; CAA54388.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S53319; S53319.

3D structure databases

ModBase

Q24803.

Family and domain databases

InterPro

IPR016162; Ald_DHase_N.
IPR012079; Bifunc_Ald/AlcDHase.
IPR001670; Fe_AlcDHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000111; ALDH_ADH; 1.

PROSITE

PS00913; ADH_IRON_1; 1.
PS00060; ADH_IRON_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Iron; Multifunctional enzyme; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 870`	`870`	`Aldehyde-alcohol dehydrogenase 2.`	`PRO_0000087835`
`NP_BIND`	`431 436`	`6`	`NAD (Potential).`
`COMPBIAS`	`54 59`	`6`	`Poly-Ala.`
`ACT_SITE`	`252 252`		`By similarity.`
`CONFLICT`	`18 18`		`R -> A (in Ref. 2; CAA54388).`
`CONFLICT`	`380 380`		`R -> E (in Ref. 1; AA sequence).`
`CONFLICT`	`382 382`		`H -> Q (in Ref. 1; AA sequence).`
`CONFLICT`	`387 388`		`HS -> NP (in Ref. 1; AA sequence).`
`CONFLICT`	`482 482`		`A -> R (in Ref. 2; CAA54388).`
`CONFLICT`	`534 534`		`T -> I (in Ref. 2; CAA54388).`
`CONFLICT`	`566 566`		`W -> R (in Ref. 2; CAA54388).`
`CONFLICT`	`739 739`		`A -> G (in Ref. 2; CAA54388).`
`CONFLICT`	`821 821`		`K -> Q (in Ref. 2; CAA54388).`

Sequence information

Length: 870 AA [This is the length of the unprocessed precursor]

Molecular weight: 95659 Da [This is the MW of the unprocessed precursor]

CRC64: 66733AD08CE3D0A5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTQQTMTVD EHINQLVRKA QVALKEYLKP EYTQEKIDYI VKKASVAALD QHCALAAAAV 

        70         80         90        100        110        120 
EETGRGIFED KATKNIFACE HVTHEMRHAK TVGIINVDPL YGITEIAEPV GVVCGVTPVT 

       130        140        150        160        170        180 
NPTSTAIFKS LISIKTRNPI VFSFHPSALK CSIMAAKIVR DAAIAAGAPE NCIQWIEFGG 

       190        200        210        220        230        240 
IEASNKLMNH PGVATILATG GNAMVKAAYS SGKPALGVGA GNVPTYIEKT CNIKQAANDV 

       250        260        270        280        290        300 
VMSKSFDNGM ICASEQAAII DKEIYDQVVE EMKTLGAYFI NEEEKAKLEK FMFGVNAYSA 

       310        320        330        340        350        360 
DVNNARLNPK CPGMSPQWFA EQVGIKVPED CNIICAVCKE VGPNEPLTRE KLSPVLAILK 

       370        380        390        400        410        420 
AENTQDGIDK AEAMVEFNGR GHSAAIHSND KAVVEKYALT MKACRILHNT PSSQGGIGSI 

       430        440        450        460        470        480 
YNYIWPSFTL GCGSYGGNSV SANVTYHNLL NIKRLADRRN NLQWFRVPPK IFFEPHSIRY 

       490        500        510        520        530        540 
LAELKELSKI FIVSDRMMYK LGYVDRVMDV LKRRSNEVEI EIFIDVEPDP SIQTVQKGLA 

       550        560        570        580        590        600 
VMNTFGPDNI IAIGGGSAMD AAKIMWLLYE HPEADFFAMK QKFIDLRKRA FKFPTMGKKA 

       610        620        630        640        650        660 
RLICIPTTSG TGSEVTPFAV ISDHETGKKY PLADYSLTPS VAIVDPMFTM SLPKRAIADT 

       670        680        690        700        710        720 
GLDVLVHATE AYVSVMANEY TDGLAREAVK LVFENLLKSY NGDLEAREKM HNAATIAGMA 

       730        740        750        760        770        780 
FASAFLGMDH SMAHKVGAAF HLPHGRCVAV LLPHVIRYNG QKPRKLAMWP KYNFYKADQR 

       790        800        810        820        830        840 
YMELAQMVGL KCNTPAEGVE AFAKACEELM KATETITGFK KANIDEAAWM SKVPEMALLA 

       850        860        870 
FEDQCSPANP RVPMVKDMEK ILKAAYYPIA

Q24803 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools