[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Bristol N2; DOI=10.1093/nar/27.17.3424; PubMed=10446229 [NCBI, ExPASy, EBI, Israel, Japan] Hough R.F., Lingam A.T., Bass B.L.; "Caenorhabditis elegans mRNAs that encode a protein similar to ADARs derive from an operon containing six genes."; Nucleic Acids Res. 27:3424-3432(1999).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Bristol N2; DOI=10.1126/science.282.5396.2012; PubMed=9851916 [NCBI, ExPASy, EBI, Israel, Japan] The C. elegans sequencing consortium; "Genome sequence of the nematode C. elegans: a platform for investigating biology."; Science 282:2012-2018(1998).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).
SUBUNIT: Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the complex I 23 kDa subunit family.
SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF140272; AAD34863.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00037; AAA50662.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

T16914; T16914.

NP_498595.1; -.

3D structure databases

HSSP

P00198; 2FDN. [HSSP ENTRY / PDB]

ModBase

Q22619.

Protein-protein interaction databases

DIP

DIP:24953N; -.

Organism-specific databases

WormBase

WBGene00020636; T20H4.5.

WormPep

T20H4.5; CE00832. [WormPep / WorfDB]

Gene expression databases

ArrayExpress

Q22619; -.

Ontologies

GO:0009792;	Biological process: embryonic development ending in birth or egg hatching (inferred from mutant phenotype from WormBase).
GO:0002119;	Biological process: nematode larval development (inferred from mutant phenotype from WormBase).
GO:0040010;	Biological process: positive regulation of growth rate (inferred from mutant phenotype from WormBase).
QuickGo view.

Family and domain databases

InterPro

IPR001450; 4Fe4S_Fe_S_bd.
IPR010226; NADH_quinone_OxRdtase_I.
Graphical view of domain structure.

Pfam

PF00037; Fer4; 2.
Pfam graphical view of domain structure.

PRINTS

PR00353; 4FE4SFRDOXIN.

TIGRFAMs

TIGR01971; NuoI; 1.

PROSITE

PS00198; 4FE4S_FER_1; 2.
PS51379; 4FE4S_FER_2; 2.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q22619.

Genome annotation databases

Ensembl

T20H4.5; Caenorhabditis elegans. [Contig view]

GeneID

176023; -.

KEGG

cel:T20H4.5; -.

NMPDR

fig|6239.3.peg.10480; -.

Other

ProtoNet

Q22619.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Repeat; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (By similarity).`
`CHAIN`	`? 212`		`Probable NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial.`	`PRO_0000020015`
`DOMAIN`	`104 133`	`30`	`4Fe-4S ferredoxin-type 1.`
`DOMAIN`	`143 172`	`30`	`4Fe-4S ferredoxin-type 2.`
`METAL`	`113 113`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`116 116`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`119 119`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`123 123`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`152 152`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`155 155`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`158 158`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`162 162`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`

Sequence information

Length: 212 AA [This is the length of the unprocessed precursor]

Molecular weight: 23870 Da [This is the MW of the unprocessed precursor]

CRC64: F50C413D89E80AB7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAMKSVAVLT KGMFGRIPAQ LAVSSVPSNT IQKRSNYKFV GMPNETDGTL AGDLNYGLHN 

        70         80         90        100        110        120 
VFFTELFRGF GVMLGHVFME PATINYPFEK GPLSSRFRGE HALRRYPSGE ERCIACKLCE 

       130        140        150        160        170        180 
AICPAQAITI EAETRPDGSR RTTRYDIDMT KCIYCGLCQE ACPVDAIVEG PNFEYSTETH 

       190        200        210 
EELLYNKEKL LLNGDRWEPE LASNLQAEYL YR

Q22619 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools