NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=U-51;
TISSUE=Gametophyte;
Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T., Kobayashi M., Okazaki T., Ohara I., Nakayama I.;
"Whole genome sequence of Porphyra yezoensis chloroplast.";
Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SUBCELLULAR LOCATION: Plastid, chloroplast.
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AP006715; BAE92402.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_536959.1; -.

3D structure databases

ModBase

Q1XDK9.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

Q1XDK9.

Genome annotation databases

GeneID

3978978; -.

Other

ProtoNet

Q1XDK9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis; Plastid.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 349`	`349`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_0000277208`

Sequence information

Length: 349 AA [This is the length of the unprocessed precursor]

Molecular weight: 41532 Da [This is the MW of the unprocessed precursor]

CRC64: 4149118183E776B9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQTTINNGQT SSKETLLTPR FYTTDFEEMA NMDISGNQED FLAILEEFRA DYNSEHFIRD 

        70         80         90        100        110        120 
EEFNQSWSNL EHKTKSLFIE FLERSCTAEF SGFLLYKELS RKLKDRNPVI AECFLLMSRD 

       130        140        150        160        170        180 
EARHAGFLNK AIGDFNLSLD LGFLTKSRKY TFFSPKFIFY ATYLSEKIGY WRYITIYRHL 

       190        200        210        220        230        240 
EQHPEHRIYP IFRFFENWCQ DENRHGDFFA ALLKSQPHFL NDWKAKMWCR FFLLSVFATM 

       250        260        270        280        290        300 
YLNDFQRIDF YNAIGLDSRQ YDMQVIRKTN ESAARVFPVA LDVDNPKFFK YLDTCACDNR 

       310        320        330        340 
ALIDIDNNNS PLFIKSIVKI PLYFSLFANL LKIYLIKPID SKTVWNTVR

Q1XDK9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools