NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1371/journal.pbio.0040188; PubMed=16729848 [NCBI, ExPASy, EBI, Israel, Japan]
Wu D., Daugherty S.C., Van Aken S.E., Pai G.H., Watkins K.L., Khouri H., Tallon L.J., Zaborsky J.M., Dunbar H.E., Tran P.L., Moran N.A., Eisen J.A.;
"Metabolic complementarity and genomics of the dual bacterial symbiosis of sharpshooters.";
PLoS Biol. 4:1079-1092(2006).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000238; ABF13980.1; ALT_INIT; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_588955.1; -.

3D structure databases

ModBase

Q1LSV9.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0033739;	Molecular function: queuine synthase activity (inferred from electronic annotation from EC).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

Q1LSV9.

Genome annotation databases

GeneID

4056410; -.

GenomeReviews

CP000238_GR; BCI_0525.

KEGG

bci:BCI_0525; -.

Phylogenomic databases

HOGENOM

Q1LSV9; -.

Genome annotation databases

CMR

Q1LSV9; BCI_0525.

Other

ProtoNet

Q1LSV9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 283`	`283`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000247702`

Sequence information

Length: 283 AA [This is the length of the unprocessed precursor]

Molecular weight: 33067 Da [This is the MW of the unprocessed precursor]

CRC64: F23E4B62CCF4A683 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTDQQYQLLQ KFKLDKSKIS YRTHYNPMLL QPIPRTIHRE LLGISSISLP FQGADFWTLY 

        70         80         90        100        110        120 
ELSWLNKYGL PQVAIGEMII DATSDNLIES QSLKLYLNSI NQTCFSSEQE LRNTLITDIT 

       130        140        150        160        170        180 
KCINSQVKIT IHSLSQWMTV PILEFTGECI DNQSIQIDND NYIFNSNLLV NSVEREKVKE 

       190        200        210        220        230        240 
TLVSHLLKSN CPITNQPDWG SVQISYYGMR INREALLRYL ISFRKYKIFH EQCVEQIYCD 

       250        260        270        280 
IMQFCLPNTL SVYVRYNRRG GLDINPWRSN ISYSPVNRPL ARQ

Q1LSV9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools