ID   OXDD1_CAEEL             Reviewed;         334 AA.
AC   Q19564; Q08I99; Q8I7K8;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-NOV-2008, entry version 53.
DE   RecName: Full=D-aspartate oxidase 1;
DE            Short=DASOX 1;
DE            EC=1.4.3.1;
DE   AltName: Full=DDO 1;
GN   ORFNames=F18E3.7;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=Bristol N2;
RX   PubMed=17140416; DOI=10.1111/j.1742-4658.2006.05571.x;
RA   Katane M., Seida Y., Sekine M., Furuchi T., Homma H.;
RT   "Caenorhabditis elegans has two genes encoding functional D-aspartate
RT   oxidases.";
RL   FEBS J. 274:137-149(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE
RP   SPLICING.
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Selectively catalyzes the oxidative deamination of D-
CC       aspartate and its N-methylated derivative, N-methyl D-aspartate.
CC   -!- CATALYTIC ACTIVITY: D-aspartate + H(2)O + O(2) = oxaloacetate +
CC       NH(3) + H(2)O(2).
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.38 mM for D-Asp;
CC         KM=0.25 mM for D-Glu;
CC         KM=1.84 mM for NMDA;
CC         Vmax=4.40 umol/min/mg enzyme with D-Asp as substrate;
CC         Vmax=3.03 umol/min/mg enzyme with D-Glu as substrate;
CC         Vmax=4.31 umol/min/mg enzyme with NMDA as substrate;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a;
CC         IsoId=Q19564-1; Sequence=Displayed;
CC         Note=No experimental confirmation available;
CC       Name=b;
CC         IsoId=Q19564-2; Sequence=VSP_014356, VSP_014357, VSP_014358,
CC                                  VSP_014359;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the DAMOX/DASOX family.
CC   -!- CAUTION: The conserved active site Tyr residue in position 224 is
CC       replaced by a Phe.
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DR   EMBL; AB275892; BAF34315.1; -; mRNA.
DR   EMBL; U53139; AAK18939.1; -; Genomic_DNA.
DR   EMBL; U53139; AAN84824.1; -; Genomic_DNA.
DR   PIR; T29219; T29219.
DR   RefSeq; NP_504908.1; -.
DR   UniGene; Cel.4023; -.
DR   HSSP; P00371; 1AN9.
DR   Ensembl; F18E3.7; Caenorhabditis elegans.
DR   GeneID; 179130; -.
DR   KEGG; cel:F18E3.7; -.
DR   NMPDR; fig|6239.3.peg.18465; -.
DR   WormBase; WBGene00017565; F18E3.7.
DR   WormPep; F18E3.7a; CE07083.
DR   WormPep; F18E3.7b; CE32864.
DR   NextBio; 904050; -.
DR   ArrayExpress; Q19564; -.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0003884; F:D-amino-acid oxidase activity; IEA:InterPro.
DR   GO; GO:0008445; F:D-aspartate oxidase activity; IEA:EC.
DR   GO; GO:0009792; P:embryonic development ending in birth or eg...; IMP:WormBase.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006181; D-amino_acid_oxidase_CS.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01266; DAO; 1.
DR   PROSITE; PS00677; DAO; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; FAD; Flavoprotein;
KW   Oxidoreductase.
FT   CHAIN         1    334       D-aspartate oxidase 1.
FT                                /FTId=PRO_0000162772.
FT   NP_BIND       8     22       FAD (Potential).
FT   ACT_SITE    305    305       By similarity.
FT   VAR_SEQ       1    222       Missing (in isoform b).
FT                                /FTId=VSP_014356.
FT   VAR_SEQ     223    228       TFTIPK -> MCQIFR (in isoform b).
FT                                /FTId=VSP_014357.
FT   VAR_SEQ     268    273       EPKIIK -> VRISFF (in isoform b).
FT                                /FTId=VSP_014358.
FT   VAR_SEQ     274    334       Missing (in isoform b).
FT                                /FTId=VSP_014359.
SQ   SEQUENCE   334 AA;  37608 MW;  90B1731A702BB6FD CRC64;
     MANIIPKIAI IGEGVIGCTS ALQISKAIPN AKITVLHDKP FKKSCSAGPA GLFRIDYEEN
     TEYGRASFAW FSHLYRTTKG SETGVKLVSG HIQSDNLESL KQQQRAYGDI VYNFRFLDDR
     ERLDIFPEPS KHCIHYTAYA SEGNKYVPYL KNLLLEQKIE FKQQEVTSLD AVADAGYDVI
     VNCAGLYGGK LAGDDDTCYP IRGVILEVDA PWHKHFNYRD FTTFTIPKEH SVVVGSTKQD
     NRWDLEITDE DRNDILKRYI ALHPGMREPK IIKEWSALRP GRKHVRIEAQ KRTSVGNSKD
     YMVVHHYGHG SNGFTLGWGT AIEATKLVKT ALGL
//