ID   HOT_AEDAE               Reviewed;         462 AA.
AC   Q17EN4;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   25-NOV-2008, entry version 22.
DE   RecName: Full=Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial;
DE            Short=HOT;
DE            EC=1.1.99.24;
DE   Flags: Precursor;
GN   ORFNames=AAEL003729;
OS   Aedes aegypti (Yellowfever mosquito).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Aedes; Stegomyia.
OX   NCBI_TaxID=7159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Liverpool;
RX   PubMed=17510324; DOI=10.1126/science.1138878;
RA   Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA   Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M.,
RA   Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J.,
RA   Sinkins S.P., Hogenkamp D.G., Amedeo P., Arensburger P.,
RA   Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V.,
RA   Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B.,
RA   DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M.,
RA   Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H.,
RA   Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S.,
RA   Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D.,
RA   Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A.,
RA   Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA   Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA   Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA   Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D.,
RA   White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A.,
RA   Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J.,
RA   Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B.,
RA   Fraser-Liggett C.M., Severson D.W.;
RT   "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL   Science 316:1718-1723(2007).
CC   -!- FUNCTION: Catalyzes the cofactor-independent reversible oxidation
CC       of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA)
CC       coupled to reduction of 2-ketoglutarate (2-KG) to D-2-
CC       hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a
CC       substrate for HOT when using 2-KG as hydrogen acceptor, resulting
CC       in the formation of D-2-HG (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate =
CC       acetoacetate + (R)-2-hydroxyglutarate.
CC   -!- CATALYTIC ACTIVITY: 2-oxoglutaric acid + 4-hydroxybutanoic acid =
CC       (R)-2-hydroxyglutaric acid + succinic semialdehyde.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. Hydroxyacid-oxoacid transhydrogenase subfamily.
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DR   EMBL; CH477280; EAT44958.1; -; Genomic_DNA.
DR   RefSeq; XP_001664064.1; -.
DR   UniGene; Aae.6969; -.
DR   GeneID; 5578877; -.
DR   KEGG; aag:AaeL_AAEL003729; -.
DR   VectorBase; AAEL003729; Aedes aegypti.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0047988; F:hydroxyacid-oxoacid transhydrogenase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0015993; P:molecular hydrogen transport; ISS:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001670; Fe_AlcDHase.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; FALSE_NEG.
DR   PROSITE; PS00060; ADH_IRON_2; FALSE_NEG.
PE   3: Inferred from homology;
KW   Mitochondrion; Oxidoreductase; Transit peptide.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    462       Probable hydroxyacid-oxoacid
FT                                transhydrogenase, mitochondrial.
FT                                /FTId=PRO_0000323003.
SQ   SEQUENCE   462 AA;  50140 MW;  294DCCE1FDF80BA1 CRC64;
     MMSKARISSL MKVISSGSCS CPAHSRSSTG GLNVSSENEY AFEMSSSTIR YGPGVSKELG
     HDLQNLNAKN VCIVTDRNVA KLNSVKVAFD SLTRCGIQYQ VYDETRVEPT DQSLLHAAEY
     ARQNKFDSFV AIGGGSVIDT CKVANLFSAD REAEFLDYVN APIGKAKEVN VKLKPLIAVP
     TTAGTGSETT GVVIFDYKPL HAKTGISSKY LRPQLGLIDP LHTLSQPEKV AAYCGFDVFC
     HALESFTAIP YTERGSAPMN PNLRPPYQGS NPISDVWARF ALKIIRENFI SAVFNQDDLK
     ARSNMHLAST MAGVGFGNAG VHLCHGLSYP ISGHVKKFVP DGYSGDHPII PHGLSVVMTA
     PAVFKFTAAS CPERHLEAAE LLGANVSKAN RNDAGSILSD VVRQYMYKLK IENGLDSLGF
     TRDDIQSLVK GTLPQERITK LAPRAQTEED LASLFENSMT VY
//