ID   ERG25_HUMAN             Reviewed;         293 AA.
AC   Q15800; Q32Q24;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   16-DEC-2008, entry version 72.
DE   RecName: Full=C-4 methylsterol oxidase;
DE            EC=1.14.13.72;
DE   AltName: Full=Methylsterol monooxygenase;
GN   Name=SC4MOL; Synonyms=DESP4, ERG25;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Intestine;
RX   MEDLINE=96279274; PubMed=8663358; DOI=10.1074/jbc.271.28.16927;
RA   Li L., Kaplan J.;
RT   "Characterization of yeast methyl sterol oxidase (ERG25) and
RT   identification of a human homologue.";
RL   J. Biol. Chem. 271:16927-16933(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Retina;
RA   Herrmann K.;
RL   Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol +
CC       NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-
CC       cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-
CC       cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-
CC       methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2
CC       H(2)O.
CC   -!- CATALYTIC ACTIVITY: 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-
CC       7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4-
CC       beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+)
CC       + H(2)O.
CC   -!- COFACTOR: Iron (Probable).
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol
CC       from lanosterol: step 3/6.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein (Probable).
CC   -!- DOMAIN: The histidine box domains may contain the active site
CC       and/or be involved in metal ion binding.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family.
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DR   EMBL; U60205; AAC50587.1; -; mRNA.
DR   EMBL; U93162; AAB81566.1; -; mRNA.
DR   EMBL; BC010653; AAH10653.1; -; mRNA.
DR   EMBL; BC107879; AAI07880.1; -; mRNA.
DR   RefSeq; NP_001017369.1; -.
DR   RefSeq; NP_006736.1; -.
DR   UniGene; Hs.105269; -.
DR   IntAct; Q15800; 1.
DR   PRIDE; Q15800; -.
DR   Ensembl; ENSG00000052802; Homo sapiens.
DR   GeneID; 6307; -.
DR   KEGG; hsa:6307; -.
DR   GeneCards; GC04P166468; -.
DR   H-InvDB; HIX0004617; -.
DR   HGNC; HGNC:10545; SC4MOL.
DR   MIM; 607545; gene.
DR   PharmGKB; PA34955; -.
DR   HOGENOM; Q15800; -.
DR   HOVERGEN; Q15800; -.
DR   Reactome; REACT_602; Lipid and lipoprotein metabolism.
DR   DrugBank; DB00157; NADH.
DR   LinkHub; Q15800; -.
DR   NextBio; 24483; -.
DR   ArrayExpress; Q15800; -.
DR   CleanEx; HS_SC4MOL; -.
DR   GermOnline; ENSG00000052802; Homo sapiens.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; TAS:ProtInc.
DR   GO; GO:0005886; C:plasma membrane; TAS:ProtInc.
DR   GO; GO:0000254; F:C-4 methylsterol oxidase activity; TAS:ProtInc.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006631; P:fatty acid metabolic process; TAS:ProtInc.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006088; Sterol_desatur.
DR   Pfam; PF01598; Sterol_desat; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Iron; Lipid synthesis; Membrane; NAD;
KW   Oxidoreductase; Polymorphism; Steroid biosynthesis;
KW   Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    293       C-4 methylsterol oxidase.
FT                                /FTId=PRO_0000117033.
FT   TRANSMEM     55     75       Potential.
FT   TRANSMEM    100    120       Potential.
FT   TRANSMEM    199    219       Potential.
FT   MOTIF       157    161       Histidine box-1.
FT   MOTIF       170    174       Histidine box-2.
FT   MOTIF       249    255       Histidine box-3.
FT   VARIANT     124    124       N -> S (in dbSNP:rs34499452).
FT                                /FTId=VAR_048898.
SQ   SEQUENCE   293 AA;  35216 MW;  D88E0DDBE85DE0BF CRC64;
     MATNESVSIF SSASLAVEYV DSLLPENPLQ EPFKNAWNYM LNNYTKFQIA TWGSLIVHEA
     LYFLFCLPGF LFQFIPYMKK YKIQKDKPET WENQWKCFKV LLFNHFCIQL PLICGTYYFT
     EYFNIPYDWE RMPRWYFLLA RCFGCAVIED TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF
     GMEAEYAHPL ETLILGTGFF IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL
     IPFYAGSRHH DFHHMNFIGN YASTFTWWDR IFGTDSQYNA YNEKRKKFEK KTE
//